PREPARATION AND CHARACTERlZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN HEMOGLOBIN

Wang Kaizheng; et al

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PREPARATION AND CHARACTERlZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN HEMOGLOBIN

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- PREPARATION AND CHARACTERlZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN HEMOGLOBIN
- Journal of Chongqing Medical University Issue 2, (1994)
- 作者机构：
  
  1. 北民佑安医院
  2. 沪州医学院附属医院
  3. 北民佑安医院沪州医学院附属医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.11
- Published：1994，
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[1]王开正,罗春丽, 陈祖贵,闵福援,冷强.抗人Hb单克隆抗体的制备与鉴定[J].重庆医科大学学报,1994(02):90-93.

Wang Kaizheng, et al. PREPARATION AND CHARACTERlZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN HEMOGLOBIN. [J]. Journal of Chongqing Medical University (2).(1994)
[1]王开正,罗春丽, 陈祖贵,闵福援,冷强.抗人Hb单克隆抗体的制备与鉴定[J].重庆医科大学学报,1994(02):90-93. DOI：

Wang Kaizheng, et al. PREPARATION AND CHARACTERlZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN HEMOGLOBIN. [J]. Journal of Chongqing Medical University (2).(1994) DOI：

摘要

用超声波快速乳化人Ｈｂ，作为抗原免疫ＢＡＬＢ／Ｃ小鼠，取其脾细胞与Ｓｐ２／０骨髓瘤细胞经离心ＰＥＧ法融合，微波ＥＬＩＳＡ筛选阳性克隆，获得三株分泌针对人Ｈｂ不同抗原位点的单克隆抗体杂交瘤细胞系。分别命名为Ｈｂ２６４、Ｈｂ２１０和Ｈｂ２３８。其中Ｈｂ２６４能特异识别人Ｈｂ的β链而不与动物Ｈｂ交叉反应；解离常数Ｋｂ为１．６×１０￣（－８）ｍｏｌ／Ｌ，ＩｇＧ＿（２ａ）。Ｈｂ２１０能结合人Ｈｂ的α链，可被猪Ｈｂ抑制，但亲合力低１００倍；Ｋｄ（人Ｈｂ）为２．８×１０￣（－８）ｍｏｌ／Ｌ；Ｋｄ（猪Ｈｂ）为３．０×１０￣（－４）ｍｏｌ／Ｌ；ＩｇＧ＿（２ｂ）。Ｈｂ２３８是两个脾细胞与一个Ｓｐ２／０细胞融合而成的三体瘤（ｔｒｉｏｍａ）；染色体１３２条；Ｈｂ２３８单克隆抗体可同人Ｈｂ的α和β链结合，Ｋｄ（人Ｈｂ）为８．９×１０￣（－５）ｍｏｌ／Ｌ；ＩｇＧ＿（２ａ）；经分析鉴定，Ｈｂ２３８是针对人Ｈｂα和β链的双特异性抗体。本方法对免疫学隐血检测、法医学鉴定和双特异抗体的研制有一定意义。

Abstract

hree hybridoma cell lines

desingnated Hb264

Hb210 and Hb238

were prepared by thedeveloped hybridomas technology. Hb264 McAb specific for the beta-chain of human Hb is themurine IgG2a

the Kd of the complex of Hb264-Hb is 1.6 mol/L

Hb210 McAb reacts to thealpha-chain of human Hb

Kd of Hb210-Hb is 2.8 mol/L; murine IgG2b.Hb238.murine IgG2a

is abispecific McAb which can combine with the alpha-chain as wdll as beta-chain of Hb;The hybridomaHb238 1s a trioma fused with two spleen cells of the mice and one Sp2/0 plasmacytoma cell;Kd of Hb238-Hb is 8.9 mol/L. The methods and results of cell fusion

screening

characterization have beendiscussed.

关键词

血红蛋白杂交瘤双特异性单克隆抗体ELISA

Keywords

hemoglobinhybridomasbispecific monoclonal antibodiesELISA

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