new technique for isolation and purification of fructose- 1

6- diphosphate（FDP）from the fermentation mixture has been reported

Which utilizes precipitation

absorptionand recrystallization instead of the reverse osmosis system or Chromatographic oncationic and anionic resins

molecular fiItration and lyophilization

Thus

4L of a fermen-tion anionic was deproteinized and precipItated usifig 50%（W/W）CaCl

2

Solution;FDPwas purified by ion exchange

microencapsulated charcoal treatment and recrystallization.The overall yield of FDP was 80%and the purity was 99%. The new technique short-ened the production period and was easy to scale up for industrial application.