METHODOLOGICAL RESEARCH FOR THE ANALYSIS OF FATTY ACIDS OF HUMAN ERYTHROCYTE MEMBRANE BY HPLC

Yang Gangyi; et al

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METHODOLOGICAL RESEARCH FOR THE ANALYSIS OF FATTY ACIDS OF HUMAN ERYTHROCYTE MEMBRANE BY HPLC

更新时间：2024-01-17

- METHODOLOGICAL RESEARCH FOR THE ANALYSIS OF FATTY ACIDS OF HUMAN ERYTHROCYTE MEMBRANE BY HPLC
- Journal of Chongqing Medical University Issue 1, (1996)
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  CLC： Q503
- Published：1996，
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[1]杨刚毅,邱鸿鑫,邓华聪,汪恕萍,张晓萍.高效液相色谱法分离人红细胞膜脂肪酸的研究[J].重庆医科大学学报,1996(01):10-12.

Yang Gangyi, et al. METHODOLOGICAL RESEARCH FOR THE ANALYSIS OF FATTY ACIDS OF HUMAN ERYTHROCYTE MEMBRANE BY HPLC. [J]. Journal of Chongqing Medical University (1).(1996)
[1]杨刚毅,邱鸿鑫,邓华聪,汪恕萍,张晓萍.高效液相色谱法分离人红细胞膜脂肪酸的研究[J].重庆医科大学学报,1996(01):10-12. DOI：

Yang Gangyi, et al. METHODOLOGICAL RESEARCH FOR THE ANALYSIS OF FATTY ACIDS OF HUMAN ERYTHROCYTE MEMBRANE BY HPLC. [J]. Journal of Chongqing Medical University (1).(1996) DOI：

摘要

本文建立了用高效液相色谱法（ＨＰＬＣ）分离人红细胞膜脂肪酸的方法。选用２－溴苯乙酮为衍生剂、１８－冠醚－６为催化剂，有Ｇ＿（１８）反相柱分离，流动相采用甲醇－乙腈－水，正十六酸（Ｃ＿（１７）：０）为内标，紫外测定波长为２５４ｎｍ，于６０ｍｉｎ内分离了人红细胞膜含有的八种主要脂肪酸。

Abstract

high-performance liquid chromatographic method was established for examining fatty acids in human ery-throcyte membrane.The fatty acids were derivatized with 2-bromoacetophenone The reaction was catalyzed by18-Crown-6.The fatty acid esters wer analysed on a C18 reversed-phase‘column with methanol-acetonitrile-water as a mobile phase and heptadecanoic acid(17:0)as the internal standard.The compounds、vere detected at 254mm.The eight kinds of fatty acids in human erythrocyte membrane were separated within 60 min.

关键词

高效液相色谱法（HPLC）红细胞膜脂肪酸

Keywords

HPLCErythrocyte membarneFatty acids

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