Determiation of Lomefloxacin In Serum And Urine By Reversed  phase HPLC

Yang Jian; et al

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Determiation of Lomefloxacin In Serum And Urine By Reversed phase HPLC

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- Determiation of Lomefloxacin In Serum And Urine By Reversed phase HPLC
- Journal of Chongqing Medical University Issue 2, (1995)
- 作者机构：
  
  第一医院传染科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R927.2
- Published：1995，
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[1]杨健,杜继昭,钱元恕,王其南.反相高效液相色谱法测定洛美沙星的血和尿药浓度[J].重庆医科大学学报,1995(02):79-81.

Yang Jian, et al. Determiation of Lomefloxacin In Serum And Urine By Reversed phase HPLC. [J]. Journal of Chongqing Medical University (2).(1995)
[1]杨健,杜继昭,钱元恕,王其南.反相高效液相色谱法测定洛美沙星的血和尿药浓度[J].重庆医科大学学报,1995(02):79-81. DOI：

Yang Jian, et al. Determiation of Lomefloxacin In Serum And Urine By Reversed phase HPLC. [J]. Journal of Chongqing Medical University (2).(1995) DOI：

摘要

本文报道用反相高效液相色谱法测定血清和尿液中洛美沙星浓度，固定相用Ｃ＿（１８）柱，流动相为甲醇／０．００８Ｍ磷酸盐缓冲液／０．５Ｍ四丁基溴化铵（２５：７５：４，Ｖ／Ｖ／Ｖ）．荧光检测激发波长２８０ｎｍ，发射波长４５５ｎｍ，以外标法定量。洛美沙星的最低检出限为０．０６μｇ／ｍｌ，线性范围为０．１２５－８．μｇ／ｍｌ．线性关系良好（ｒ＝０．９９９９），日内变异系数２．８７％，日间变异系数２．９４％，回收率７５．８％～８０．９％。

Abstract

Reversed-phase HPLC was described for the separation and determination of Iomefloxacin inserum and urine. Samples were analysed on an ODS column (7μ

250×4.6mm) with a mobile phase ofmethand-0.008M phosphate buffer-0.5M tetrabuty lammonium bromide(25: 75 :4

v/v/v).usingfluorescence detection at λ ex 280nm and λ em 455nm.The detection limit was 0. 06μg/ml. GoodIinearity was obtained in the concentration range from 0. 125 to 8μg/ml with the correlation coeffi-cient r= 0. 9999. The average recovery was 75.8%-80.9%and the differences within and betweendays were 2. 87%

2. 94% respectively

关键词

高效液相色谱法洛美沙星

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