HIGH LEVEL EXPEESSION Bordetella Pertussis TOXIN s3 SUBUNT BY THE FUSING PROTEIN TECHNIQUE IN Escherichia coli

Yin Yibing

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HIGH LEVEL EXPEESSION Bordetella Pertussis TOXIN s3 SUBUNT BY THE FUSING PROTEIN TECHNIQUE IN Escherichia coli

更新时间：2024-01-17

- HIGH LEVEL EXPEESSION Bordetella Pertussis TOXIN s3 SUBUNT BY THE FUSING PROTEIN TECHNIQUE IN Escherichia coli
- Journal of Chongqing Medical University Issue 2, (1996)
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  CLC： R378.42
- Published：1996，
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[1]尹一兵.用融合蛋白在大肠杆菌中高表达百日咳杆菌毒素蛋白S＿3亚基基因[J].重庆医科大学学报,1996(02):103-106.

Yin Yibing. HIGH LEVEL EXPEESSION Bordetella Pertussis TOXIN s3 SUBUNT BY THE FUSING PROTEIN TECHNIQUE IN Escherichia coli. [J]. Journal of Chongqing Medical University (2).(1996)
[1]尹一兵.用融合蛋白在大肠杆菌中高表达百日咳杆菌毒素蛋白S＿3亚基基因[J].重庆医科大学学报,1996(02):103-106. DOI：

Yin Yibing. HIGH LEVEL EXPEESSION Bordetella Pertussis TOXIN s3 SUBUNT BY THE FUSING PROTEIN TECHNIQUE IN Escherichia coli. [J]. Journal of Chongqing Medical University (2).(1996) DOI：

摘要

用多聚酶链反应（ＰＣＲ）和基因重组技术通过百日咳毒素Ｓ＿３亚基基因和高表达质粒ｐＭＡＬ－Ｃ构建了麦芽糖结合蛋白（ＭＢＰ）和（Ｓ＿３）亚基蛋白的融合基因，并在大肠杆菌中高效表达了（ＭＢＰ－Ｓ＿３）融合蛋白。该融合蛋白产率占细胞总蛋白含量的２５％，并且能用蛋白酶因子Ｘａ将融会蛋白切开，得到ＭＢＰ（４２ＫＤ）和Ｓ＿３亚基蛋白（２１．８７ＫＤ）．该方法表达式效率高。纯化过程简单，为基因工程技术生产该毒素提供了新的途径。

Abstract

sing Polyinerase Chain Reaction(PCR)and recombinant DNA techniques

a fusing genevector inc luding maltose binding protein(MBP)and pertussis toxin S3(PTX-S3) subunit proteinis constructed by PTX-S3 gene and a high level expressioa plasmid pMAL-C. High levelexpressi on of the fusing protein may be produced by the fusing pla smid in E. coli

the fusingprotein (MW

63.87KD)yield is about 25% in the whole cell protein. MBP(MW

42KD) andPTX-S3(MW

21.87KD)may be obtained by cutting the fusing protein with the factor-Xa. Themethod have a high level expression and simple purification pmcess

which will be a verv usefulroad to eontribute and produce PTX-S3 in E. coli.

关键词

百日咳杆菌毒素S＿3大肠杆菌融合蛋白

Keywords

Bordetella peitussis toxin S3 subunitescherichia coliFusing protein

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