mmunobloting analysis was camed out on the virion protein of strain chen and strain R22viruses

the a eti ologic agent of EHF.The viral antigen was purified and characterized roughly byprotamine sulfate and polyethylene glycol precipition from brain tissue of suckling mice infectedwith EHFV strain chen or strain R22.Immunobloting analysis was performed withanti-EHFV sera(anti-R22 virus sera

Zhejian R3sera)and Mcab A19

A20

A35

A25-1

R31.Itshowed four bands on NC with molecular weights of 75KD

69KD

61KD

in strain chen and three bands on NC with molecular weights of 72KD

69KD

64KD in strain R22.All antibodiesreacted with the viral antigen of the two viruses.It shows a little difference between stra in clien and R22 in Virral antigen.