Application of polyaaerase chain reaction - single strand comformation polymorphiem(PCR - SSCP)in genotyping of chlamydia trachomatis

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Application of polyaaerase chain reaction - single strand comformation polymorphiem(PCR - SSCP)in genotyping of chlamydia trachomatis

更新时间：2024-01-17

- Application of polyaaerase chain reaction - single strand comformation polymorphiem(PCR - SSCP)in genotyping of chlamydia trachomatis
- Journal of Chongqing Medical University Issue 4, (1997)
- 作者机构：
  
  基础医学院微生物学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R374
- Published：1997，
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[1]夏云,朱道银.沙眼衣原体基因分型及其应用[J].重庆医科大学学报,1997(04):287-289.

Application of polyaaerase chain reaction - single strand comformation polymorphiem(PCR - SSCP)in genotyping of chlamydia trachomatis. [J]. Journal of Chongqing Medical University (4).(1997)
[1]夏云,朱道银.沙眼衣原体基因分型及其应用[J].重庆医科大学学报,1997(04):287-289. DOI：

Application of polyaaerase chain reaction - single strand comformation polymorphiem(PCR - SSCP)in genotyping of chlamydia trachomatis. [J]. Journal of Chongqing Medical University (4).(1997) DOI：

摘要

本文分别以沙眼衣原体（Ｃｈｌａｍｙｄｉａｔｒａｃｈｏｍａｔｉｓ，Ｃｔ）隐蔽性质粒引物（Ｐｐ）及主要外膜蛋白基因（ｏｍｐ；）引物（Ｐｍ）．用ＰＣＲ扩增Ｃｔ特异性ＤＮＡ，并用单链构象多态性（Ｓｉｎｇｌｅ－ｓｔｒａｎｄｃｏｎｆｏｒｍａｔｉｏｎｐｏｉｙｍｏｒｐｈ－ｉｓｍ，ＳＳＣＰ）作基因分型。４２６份标本中，９２例质粒ＰＣＲ阳性，用质粒ＰＣＲ阳性标本作ｏｍｐ：ＰＣＲ，６６例为阳性。其中５２例标本经ＳＳＣＰ分析显示７种不同的电泳图谱，１８例与标准株ＴＥ５５电泳图谱相同。其中两份标本分别取羊水及新生儿睑结膜，其ＳＳＣＰ图谱相同，表明两株Ｃｔ核酸序列完全相同。在分子水平上证实了Ｃｔ的垂直传播。临床标本可先采用敏感的质粒ＰＣＲ作初筛。阳性者再作ｏｍｐ１ＰＣＲ进行基因分型。应用ＰＣＲ－ＳＳＣＰ对Ｃｔ作基因分型，在分子流行病学研究中有重要意义。

Abstract

Rapid diagnosis of chlamydia frachomatis infection was done by using PCR to amplify C. trachozoatis specific DNA with the cryptic plasmid primer and the major outer membrane protein gene(ompl)primer seperately

Of 426 samples

92 were positive for plasmid PGR

Of plasmid PCR positive samples

66 were positive for ompl PCR. 52 samples were analyaed by SSCP and 7 diferent profiles were detected. 18 samples showed the same profile compared with TE55 reference strain. An amniotic sample and a conjunctival saznple obtained from a neonate exhibited identical BSCP profile. lt indicated that the D NA sequences of the two C.trachomatis strain were identical. and vertical transmission of C. trachomatis was confirmed in the case. When genotyping was performed by using clinical apecimens

it was suitable to screen the samples by plasmid PGR and the positive specimens were further performed by ompl PCR for genotyping. Genotyping of C. trachomatis could be perfomed by PCR - SSCP and it was important in molecular epidemiological rescarch.

关键词

沙眼衣原体基因分型单链构象多态性垂直传播

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