Construction and expression of a recombinant hepatits c virus gene vector

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Construction and expression of a recombinant hepatits c virus gene vector

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- Construction and expression of a recombinant hepatits c virus gene vector
- Journal of Chongqing Medical University Issue 2, (1998)
- 作者机构：
  
  浙江医科大学传染病研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R512.630.2
- Published：1998，
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[1]周卫平,张大志,张定凤,刘勇.重组丙型肝炎病毒基因载体的构建与表达[J].重庆医科大学学报,1998(02):133-136.

Construction and expression of a recombinant hepatits c virus gene vector. [J]. Journal of Chongqing Medical University (2).(1998)
[1]周卫平,张大志,张定凤,刘勇.重组丙型肝炎病毒基因载体的构建与表达[J].重庆医科大学学报,1998(02):133-136. DOI：

Construction and expression of a recombinant hepatits c virus gene vector. [J]. Journal of Chongqing Medical University (2).(1998) DOI：

摘要

应用基因重组技术。从载体ＣＤＺ２酶切获得１．７３ｋｂＤＮＡ片段（含１６ｄ９３ｂｐ的ＨＣＶ结构区ｃＤＮＡ，其特异性已为ｓｏｕｔｈｅｒｎｂｌｏｔ证实）。将ＨＣＶｃＵＮＡ片段插入载体ｐｃＤＮＡ３，构建ＨＣＶ重组体。经在大肠杆菌中表达，筛选、酶切分析，获得重组ＨＣＶ（ＰｃＤＮＡ３－ＨＣＶ）。以重组质粒转染Ｈ９细胞（ＣＤ淋巴细胞系），用Ｇ４１８筛选出抗性细胞。经ＲＴ－ＰＣＲ、ｄｏｔ－ＥＬＩＳＡ验证表明，ｐｃＤＮＡ３－ＨＣＶ能在Ｈ９细胞中进行复制、转录和表达。这为丙型肝炎发病机理的研究提供了一个有用模型。

Abstract

By inserting 1693bp HCV cDNA (total structure region genes) from CDZ2 into pcCNA3 vector

the recombinant HCV expressive vector pcDNA3 - HCV was constructed. To identify the recombinant HCV

it was intoduced into JM109 E.coli strain

then restriction enzyme - reaction was carried out respectively. H9 cell line(CD T cell) was transfected by the recombinant HCV expressive vector pcDNA3 - HCV and G418 resistant cell clones were identified. RT - PCR

dot ELISA showed that the pcDNA3 - HCV was replicated. transcribed and expressed in H9 cells. The construction of the H9 cell model transfixed by pcD) -- HCV was successful.

关键词

丙型肝炎病毒转染基因重组表达

Keywords

hepatitis C virusRecombinantTransfectionexpression

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