The neurotrophic effect of the periischemic brain tissue extract on culture neurons Institute of Neurology

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The neurotrophic effect of the periischemic brain tissue extract on culture neurons Institute of Neurology

更新时间：2024-01-17

- The neurotrophic effect of the periischemic brain tissue extract on culture neurons Institute of Neurology
- Journal of Chongqing Medical University Issue 2, (1998)
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  CLC： R-332;R743.3
- Published：1998，
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[1]张拥波,彭国光,董为伟.脑缺血灶周围组织提取液对培养神经元的神经营养作用[J].重庆医科大学学报,1998(02):130-132.

The neurotrophic effect of the periischemic brain tissue extract on culture neurons Institute of Neurology. [J]. Journal of Chongqing Medical University (2).(1998)
[1]张拥波,彭国光,董为伟.脑缺血灶周围组织提取液对培养神经元的神经营养作用[J].重庆医科大学学报,1998(02):130-132. DOI：

The neurotrophic effect of the periischemic brain tissue extract on culture neurons Institute of Neurology. [J]. Journal of Chongqing Medical University (2).(1998) DOI：

摘要

本文用线栓法制备鼠右侧大脑中动脉闭塞模型，分离新生鼠前脑皮质、基底节神经元进行原代培养，以维持神经元存活的总数和长出神经突出超过１００μｍ的神经元数目分别来反映脑缺血灶周围组织提取液（ＩＢＴＥ）中神经营养因子（ＮＴＦｓ）和促进神经元突起生长的神经营养因子（ＮＰＦｓ）的存在及其活性。结果显示：缺血对侧ＢＴＥ和ＩＢＴＥ维持神经元存活的总数和长出神经突超过１００μｍ的神经元数目均较空白对照组明显增加（Ｐ＜０．０１）；ＩＥＴＥ的这种作用较缺血对侧脑组织提取液的作用更为显著；随着缺血后时间的延长，ＩＢＴＥ的神经营养活性有增加趋势。结果提示ＩＢＴＥ含有维持体外培养的神经元存活（ＮＴＦｓ）和促进神经突起生长（ＮＰＦｓ）的神经营养因子的存在。这对脑缺血后神经元的损伤修复可能起重要作用。

Abstract

Focal cerebral ischemia was produced by occlusion of the right middle cerebral artery (MCA)using nylon thread. The cortex and basal neurons from newborn rats were isolated and cultured.The neurotrophic activity of brain tissus extract (BTE) was detected by counting the number of supporting neuronal survival and promoting neurite outgrowth above 100μm The results showed that the periischemic brain tissue extract supported neuronal survival and promoted neurite outgrowth. The number of neurons was significantly higher in the presence of the ischemic brain tissue extract (IBTE) as compared with the controls without BTE(p<0.01).The number was also significantly higher in the presence of IBTE as compared with the non - IBTE(contralateral BTE group).The effect of IBTE had a tendency to increase following the time after ischemia. These results suggest that the activities of NTFs might play an important role in survival of neurons after ischemia.

关键词

脑缺血神经元神经元营养因子细胞培养

Keywords

cerebral ischemianeuronNeurotrophic factorscell culture

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