Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM

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Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM

更新时间：2024-01-17

- Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM
- Journal of Chongqing Medical University Issue 4, (1997)
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  CLC： R322.47
- Published：1997，
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[1]徐勇,杨清,涂植光.荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度[J].重庆医科大学学报,1997(04):305-307.

Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM. [J]. Journal of Chongqing Medical University (4).(1997)
[1]徐勇,杨清,涂植光.荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度[J].重庆医科大学学报,1997(04):305-307. DOI：

Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM. [J]. Journal of Chongqing Medical University (4).(1997) DOI：

摘要

采用胶原酶原位灌注法分离纯化了大鼠肝细胞，并用荧光探针Ｆｕｒａ－２／ＡＭ测定了细胞内游离钙离子浓度。结果显示，胶原酶原位灌注法可分离获得高纯度，高活率的单个大鼠肝细胞，适合用荧光探针Ｆｕｒａ－２／ＡＭ测定胞内游离钙离子浓度；铡得新分离大鼠肝细胞静息状态下细胞内游离钙离子浓度为１７４．５±５７．３ｎｍｏｌ／Ｌ(我们就上述两种技术进行了一定的改进和探讨。

Abstract

The rat hepatocytes were isolated in situ with collagenase perfusion method and the cytosolic Ca~2+ levels were measured using fluorescent probe fura - 2/AM . Some improvement on the two reported methods was made. The results suggested that isolated hepatocytes were of high purity and viability

and suitable for the measurement of cytosolic Ca~2+ with fura - 2/AM. The cytosolic Ca~2+ level of isolated rat hepatocytes in metabolic quiescent stage was:174.5± 57.3 nmol/L ( s)

关键词

肝细胞钙离子荧光光度法胶原酶类灌注法

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