Cloning of B7 - 1 cDNA and its expression on leukemia cells

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Cloning of B7 - 1 cDNA and its expression on leukemia cells

更新时间：2024-01-17

- Cloning of B7 - 1 cDNA and its expression on leukemia cells
- Journal of Chongqing Medical University Issue 2, (1998)
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  CLC： R446.62
- Published：1998，
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[1]黄贵清,李成荣,杨锡强,蒋利萍,王莉佳.B细胞活化因子B7-1cDNA的克隆及其在白血病细胞的表达[J].重庆医科大学学报,1998(02):137-139.

Cloning of B7 - 1 cDNA and its expression on leukemia cells. [J]. Journal of Chongqing Medical University (2).(1998)
[1]黄贵清,李成荣,杨锡强,蒋利萍,王莉佳.B细胞活化因子B7-1cDNA的克隆及其在白血病细胞的表达[J].重庆医科大学学报,1998(02):137-139. DOI：

Cloning of B7 - 1 cDNA and its expression on leukemia cells. [J]. Journal of Chongqing Medical University (2).(1998) DOI：

摘要

利用ＲＴ－ＰＣＲ方法从ＥＢＶ转化Ｂ淋巴细胞中扩增出Ｂ７－１ｃＤＮＡ、酶切Ｂ７－１基因和逆转录病毒载体ＰＬＸＳＮ，连结、克隆ＰＬＢ７－１ＳＮ到大肠杆菌Ｍｃ１０６１。扩增纯化质粒ＰＬＢ７－１ＳＮ。用ＰＡ３１７细胞包装病毒，ＮＩＨ３Ｔ３细胞筛查病毒滴度，获得高效价的Ｂ７－１病毒上清，病毒感染人白血病细胞株Ｋ５６２和ＨＬ６０，获得稳定表达Ｂ７－１分子的肿瘤细胞。

Abstract

B7- 1 cDNA was obtained by reverse transcription polyrnerase chain reaction (RT - PCR) and recombined to retroviral vector PLXSN. Calcium phosphate - DNA precipation was used to transfect PLB7SN into packaging cells PA317 . Human leukemia cells K562 and HL60 were infected with higher titer of virus. B7 - 1 gene expression was confirmed by PCR.

关键词

B7－1基因重组逆转录病毒载体白血病细胞

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