Experimental study on the effect of proliferation and differentiation ofK562 cell treated with TSPG

Chen Tingmei; et al

您当前的位置：

首页 >

文章列表页 >

Experimental study on the effect of proliferation and differentiation ofK562 cell treated with TSPG

更新时间：2024-01-17

- Experimental study on the effect of proliferation and differentiation ofK562 cell treated with TSPG
- Journal of Chongqing Medical University Issue 2, (1999)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R733.7
- Published：1999，
扫描看全文
[1]陈婷梅,王亚平.人参总皂甙对K_562细胞增殖抑制与诱导分化的实验研究[J].重庆医科大学学报,1999(02):115-119.

Chen Tingmei, et al. Experimental study on the effect of proliferation and differentiation ofK562 cell treated with TSPG. [J]. Journal of Chongqing Medical University (2).(1999)
[1]陈婷梅,王亚平.人参总皂甙对K_562细胞增殖抑制与诱导分化的实验研究[J].重庆医科大学学报,1999(02):115-119. DOI：

Chen Tingmei, et al. Experimental study on the effect of proliferation and differentiation ofK562 cell treated with TSPG. [J]. Journal of Chongqing Medical University (2).(1999) DOI：

摘要

采用细胞体外培养，流式细胞术、形态学观察、组化与免疫细胞化学等方法，研究人参总皂甙（ＴＳＰＧ）对Ｋ＿６５２细胞增殖与分化的影响。结果表明：ＴＳＰＧ对Ｋ＿５６２细胞增殖具有明显的抑制作用，并能阻止Ｋ６５２细胞由Ｇ０／Ｇ１期向Ｇ２／Ｍ＋Ｓ期过渡：经２００μｇ／ｍｌ的ＴＳＰＧ诱导后，Ｋ５６２细胞形态趋向成熟分化；联苯胺染色、糖原染色和ＨＩＲ２．ＣＤｗ４１阳性表达细胞数显著提高，且阳性强度增加。推测ＴＳＰＧ是能抑制白血病细胞增殖并诱导其分化的天然诱导剂，但对其机理有待进一步探讨。

Abstract

By using cell culture ex.vivo

flow cytometry (FCM)

morphological observation

cytochemistry and immunocytochemistry

the effects of Total Saponins of Panax Ginseng (TSPG) on proliferation and differentiation of K562 cells were studied. The results indicated that TBPG. could markedly inhibit the proliferation of K562 cells

block K562 cells entering the active proliferative phase (G2/M+S)

and promote K562 cells to differentiate toward the mature blood cell. Our experiment also showed that after induced by TSPG

the ratio of positive K562 cells of expressing HIR2 CDw4t

glycogen

benzidine stain is obviously higher than that of control

Combining with our previous reasearcb works

we infer that TSPG may be one of the natural inducers which can not only enhance normal hematopoiesis

but also inhibit proliferation of such cancer cells as leukemia cells

and induce differentiation of these cells. The mechanism should be continually studied.

关键词

人参总皂甙K562细胞增殖诱导分化

Keywords

TSPGK562 cellsproliferationdifferentiation

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Construction of eukaryotic expression vector of open reading frame of unknown KH gene and its effect on proliferation of K562 cell

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Proteasome activator subunit 1/2 promotes the proliferation and invasion of endometrial carcinoma cells by inhibiting the expression of cyclin-dependent kinase 15

Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

Research progress of molecular mechanism of palmitic acid-induced pancreatic β-cell dysfunction

Related Author

HE Yujuan

et al

刘小珊

马凌娣

欧一衡

蒋纪恺

Liu Jie

Xie Xingming

Related Institution

Department of Clinical Biochemistry,College of Basic Medicine,Chongqing Medical University

重庆医科大学临床学院血液科

重庆市永川市人民医院

重庆医科大学基础医学院临床生化教研室

永川

⁰