Deternination  of catecholamines in plasma by high performance liquidchromatography coupled with fluorescence detection

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Deternination of catecholamines in plasma by high performance liquidchromatography coupled with fluorescence detection

更新时间：2024-01-17

- Deternination of catecholamines in plasma by high performance liquidchromatography coupled with fluorescence detection
- Journal of Chongqing Medical University Issue 3, (1997)
- 作者机构：
  
  基础医学院化学教研室中心实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： O657.72
- Published：1997，
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[1]张晚萍,王国民,邱宗荫,赵华,李惠芝.荧光-高效液相色谱法测定血浆中儿茶酚胺类化合物的研究[J].重庆医科大学学报,1997(03):194-197.

Deternination of catecholamines in plasma by high performance liquidchromatography coupled with fluorescence detection. [J]. Journal of Chongqing Medical University (3).(1997)
[1]张晚萍,王国民,邱宗荫,赵华,李惠芝.荧光-高效液相色谱法测定血浆中儿茶酚胺类化合物的研究[J].重庆医科大学学报,1997(03):194-197. DOI：

Deternination of catecholamines in plasma by high performance liquidchromatography coupled with fluorescence detection. [J]. Journal of Chongqing Medical University (3).(1997) DOI：

摘要

血浆中儿茶酚胺类化合物（ＣＡｓ）与二苯基硼酸－２－氨基乙酯在碱性条件下生成配合物，经液液提取后，与１，２－二苯基乙二胺（ＤＰＥ）在适宜的条件下发生衍生化反应，生成相应的二苯基喹喔啉衍生物。这些衍生物在岛津Ｓｈｉｍ－ＰａｃｋＣＬＣ－ＯＤＳ柱上，用甲醇－０．５０ｍｏｌ－１Ｔｒｉｓ－ＨＣＩ缓冲液等度洗脱，１６ｍｉｎ能完全分离；在Ｗａ－ｔｅｒｓＮｏｖａ－ｐａｃｋＣ１８柱上，用甲醇０．０１ｍｏｌ．Ｌ－１Ｔｒｉｓ－ＨＣＩ缓冲液等度洗脱，１０ｍｉｎ能完全分离；荧光检测波长：ｅｘ３５０ｎｍ，ｅｍ４８０ｎｍ。去甲肾上腺素（ＮＥ）、肾上腺素（Ｅ）和多巴胺（ＤＡ）的检测限分别为１０ｆｍｏｌ．１００ｕｌ－１．１０ｆｍｏｌ．１００ｕｌ－１和１２．５ｆｏｍｏｌ．１００ｕｌ－１。本方法用于正常人及疾病患者血浆中ＣＡｓ的测定，均得到满意结果。

Abstract

A senaitive fluorimetric method for the determination of catecholamines in human plasma was described. After liquid - liquid extraction with diphenylboric acid 2 - aminoethyl ester

the catecholamines in plasma are converted into the corresponding fluorescent compounds by reaction with 1

2 - diphenylethylenediamine in aqueous acetonitrile. After being isocraticly eluted by a methanol buffer solution

these compounds could completely be separated with 16 minutes on Shim - pack CLC - ODS column

and with 10 minutes on Waters Nova - pack C18 column

The limits of detection were 10 fmol for norepinephrine

10 fmol for epinephrine and 12.5 fmol for dopamine reapectively.

关键词

儿茶酚胺12－二苯基乙二胺柱前衍生法荧光高效液相色谱法

Keywords

Catecholamines12 - Diphenylethylenediamine(DPE)Precolumn derivatizationHigh performance liquid Chromatography - Fluorescence detection (HPLC - FD)

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