The optimal electroporation condition of recombinant plasmid

Zhou Zhi; et al

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The optimal electroporation condition of recombinant plasmid

更新时间：2024-01-17

- The optimal electroporation condition of recombinant plasmid
- Journal of Chongqing Medical University Issue 2, (1999)
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  CLC： Q782
- Published：1999，
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[1]周智,张定凤,任红.重组质粒电穿孔转染条件探讨[J].重庆医科大学学报,1999(02):130-132.

Zhou Zhi, et al. The optimal electroporation condition of recombinant plasmid. [J]. Journal of Chongqing Medical University (2).(1999)
[1]周智,张定凤,任红.重组质粒电穿孔转染条件探讨[J].重庆医科大学学报,1999(02):130-132. DOI：

Zhou Zhi, et al. The optimal electroporation condition of recombinant plasmid. [J]. Journal of Chongqing Medical University (2).(1999) DOI：

摘要

目的：为优化电穿孔转染质粒的条件，提高转染率。方法：以不同条件用电穿孔方法将重组质粒ＰＬＸＳＮ－Ｓ转入ｐ８１５、ｐＡ３１７、ＨｅｐＧ２、ＥＬ４等真核细胞，探讨电压，电容及电转缓冲液温度对转染率的影响。结果（１）低电压（２００～３００Ｖ），高电容（９００～１０００ｕＦ）能高效转染实验所用真核细胞；（２）冰浴可提高转染率；（３）电击时间以１５～３０ｍｓ较合适。结论：电穿孔是一种高效的基因转染方法，通过优化其条件，可以提高转染率。

Abstract

o seek for the optimal. condition and improve the rate of recombinant plasmid transfection by electroporation. Methods: PLXSN - S was transfected into several eukaryotic cell lines including p815

pA317

HepG2

El4 by electroporation and the effects of a few factors (such as voltage

capacity and the temperature of media et al) on the rate of transfection were investigated .Result (1) Low voltage (200～300v)

high capacitance (900～1000uF) is a favaorable condition for the eukaryotic cells above. (2) Ice bathing may improve the rate of transfection. (3) the optimum time is in the range of 15～30ms . Conclusion: Electroporation was a highly effective method and by optimizing the condition

the rate of transfection may be markedly improved

关键词

电穿孔基因转染真核细胞

Keywords

electroporationgene transfectionEukaryotic cell

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