PCR Cloning, the sequencing of the heavy chain variable region gene ofMcAb against HBeAg

Yang Chun; et al

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PCR Cloning, the sequencing of the heavy chain variable region gene ofMcAb against HBeAg

更新时间：2024-01-17

- PCR Cloning, the sequencing of the heavy chain variable region gene ofMcAb against HBeAg
- Journal of Chongqing Medical University Issue 1, (1999)
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  CLC： R392-33
- Published：1999，
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[1]杨春,王毅,朱道银.抗HBeAg单克隆抗体重链可变区基因的PCR法克隆和测序[J].重庆医科大学学报,1999(01):4-6.

Yang Chun, et al. PCR Cloning, the sequencing of the heavy chain variable region gene ofMcAb against HBeAg. [J]. Journal of Chongqing Medical University (1).(1999)
[1]杨春,王毅,朱道银.抗HBeAg单克隆抗体重链可变区基因的PCR法克隆和测序[J].重庆医科大学学报,1999(01):4-6. DOI：

Yang Chun, et al. PCR Cloning, the sequencing of the heavy chain variable region gene ofMcAb against HBeAg. [J]. Journal of Chongqing Medical University (1).(1999) DOI：

摘要

本文应用ＰＣＲ技术从ＨＢｅＡｇ单克隆抗体鼠─鼠杂交瘤细胞基因组ＤＮＡ中扩增出重链可变区基因（ＶＨ），并将ＶＨ基因与ｐＵＣ１９载体连接，转化ＪＭ１０９菌，克隆出ＶＨ基因。重组质粒ｐＵＣ－ＨＢｅＡｇＶＨ经荧光核酸测序表明，该ＶＨ基因大小为３２７ｂｐ，编码１０９个氨基酸。

Abstract

The VH gene ws amplified from genomic DNA of hybridomacells cells producing McAb against HBeAg byPCR reaction. The PCR products were ligated with PUC19 vector.Then VH gene was cloned by transforming itinto JM109. The recombinant plasmid pUC─HBeAgVH was sequenced by chain terination─based cyclesequencing protocal. The result shows that the VH gene was 327 base pair encoding 109 ainino acids.

关键词

重链可变区基因PCR克隆测序

Keywords

VH genePCR cloningSequencing

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Related Institution

Department of Pharmacology,College of Basic Medicine,Chongqing Medical University

第三军医大学野战外科研究所分子生物学中心

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