The mutual effect of cisplatin and 5-fluorouracil on Hela cell line

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The mutual effect of cisplatin and 5-fluorouracil on Hela cell line

更新时间：2024-01-17

- The mutual effect of cisplatin and 5-fluorouracil on Hela cell line
- Journal of Chongqing Medical University Issue 1, Pages: 45-47(2001)
- 作者机构：
  
  1. 重庆医科大学临床学院妇产科
  2. 重庆医科大学临床学院眼科
  3. 重庆医科大学病理生理学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2001，
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[1]彭勤,彭惠,骆云鹏,汤为学.顺铂、5-氟尿嘧啶对人宫颈肿瘤细胞(Hela细胞)的相互作用[J].重庆医科大学学报,2001(01):45-47+50.

The mutual effect of cisplatin and 5-fluorouracil on Hela cell line. [J]. Journal of Chongqing Medical University (1):45-47(2001)
[1]彭勤,彭惠,骆云鹏,汤为学.顺铂、5-氟尿嘧啶对人宫颈肿瘤细胞(Hela细胞)的相互作用[J].重庆医科大学学报,2001(01):45-47+50. DOI：

The mutual effect of cisplatin and 5-fluorouracil on Hela cell line. [J]. Journal of Chongqing Medical University (1):45-47(2001) DOI：

摘要

目的：观察顺铂、5-氟尿嘧啶及甲氨喋呤在体外对人宫颈肿瘤细胞（Hela细胞）的作用。并观察顺铂、5-氟尿嘧啶配伍对Hela细胞的相互作用。方法：采用MTT测定法观察顺铂、5-氟尿嘧啶及甲氨喋呤单用及合用对Hela细胞的影响，流式细胞仪观察凋亡及细胞周期的变化。并利用中效原理判定顺铂和5-氟尿嘧啶合用的效果。结果：顺铂和5-氟尿嘧啶单独应用时随着药物剂量增加，其抑制作用也增加，两药大剂量（大于中效剂量）合用时可产生拮抗作用（CI>1），小剂量（小于中效剂量）合用时可产生协同作用（CI<1）。甲氨喋呤在本实验条件下未见明显抑瘤作用。结论：顺铂和5-氟尿嘧啶合用小剂量产生协同作用，大剂量产生拮抗作用。

Abstract

Objective: To study the ability of cisplatin

5-fluorouracil and methotrexatum on inhibiting the proliferation of HeLa cell line. And to evaluate the killing mutual effects of PDD and 5-Fu on Hela cell line. Methods: To observe the effect of individual and associated drugs on HeLa cells the methyl thiazolyl tetrazolium (MTT) assay was used. The changes of cell period and the apoptosis were observed with flow cytometer (FCM) and the median-effect principle was also used. Results: Killing effects of the individual drugs were enhanced as drug concentration increased. We also observed that the interaction of drugs was at higher concentration(CI>1)

synergism at lower concentration(CI<1). but methotrexatum had no obvious effect on HeLa cells in this experimental condition. Conclusion: The higher concentration with combination was observed in antagonism

the lower concentration in synergism.

关键词

HeLa细胞抗癌药物合用指数

Keywords

HeLa cellsAnticancer drugCombination index

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