Experimental  study on DHBV DNA in duck’s  pancreas and spleen tissues by in situ hybridization

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Experimental study on DHBV DNA in duck’s pancreas and spleen tissues by in situ hybridization

更新时间：2024-01-17

- Experimental study on DHBV DNA in duck’s pancreas and spleen tissues by in situ hybridization
- Journal of Chongqing Medical University Issue 1, Pages: 60-62(2001)
- 作者机构：
  
  1. 重庆医科大学临床学院传染科,重庆,400016
  2. 重庆医科大学第二临床学院传染科,重庆,400010
  3. 重庆医科大学临床学院传染科! 重庆　400016重庆医科大学第二临床学院传染科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R341
- Published：2001，
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[1]彭凤英,郭树华.DHBV DNA在鸭胰脾组织中原位杂交的研究[J].重庆医科大学学报,2001(01):60-62.

Experimental study on DHBV DNA in duck’s pancreas and spleen tissues by in situ hybridization. [J]. Journal of Chongqing Medical University (1):60-62(2001)
[1]彭凤英,郭树华.DHBV DNA在鸭胰脾组织中原位杂交的研究[J].重庆医科大学学报,2001(01):60-62. DOI：

Experimental study on DHBV DNA in duck’s pancreas and spleen tissues by in situ hybridization. [J]. Journal of Chongqing Medical University (1):60-62(2001) DOI：

摘要

目的：对慢性DHBV感染鸭的肝、胰、脾组织 DHBV DNA作对比研究。方法：采用原位杂交技术。结果：DHBV不仅可以感染鸭肝细胞，且可感染胰、脾组织，DHBV DNA 阳性细胞的分布密度以肝组织最高，胰脾次之。结论：DHBV具有组织器官泛嗜性，但其在肝外组织中的复制似不及在肝细胞中活跃。

Abstract

Objective: To study the difference of DHBV DNA in the duck’s liver

pancreas and spleen tissue. Methods : In situ hybridization using digoxigenin-labeled DHBV DNA C gene probe. Results: DHBV DNA was present not only in hepatocytes but also in the pancreatic acini

in cells of splenic red pulp and in the splenic germinal center. The DHBV DNA positive cells appeared more frequently in liver than in spleen and pancreas. Conclusion: It suggests that DHBV is also tropic for extrahepatic tissue

but it may replicate more actively in liver than in the extrahepatic tissue.

关键词

鸭乙型肝炎病毒原位杂交DNA探针地高辛标记

Keywords

DHBVIn situ hybridizationDNA probeDigoxigenin-labeled

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