Objective: Methodology evaluation was performed for detection of ASO and RF by rate nephelometry. Methods: We determined the levels of serum RF in 158 patients and the levels of serum ASO in 96 patient serum samples and 25 normal subjects. At the same time

rate nephelometry was compared with latex agglutination test. Results: The CV value of intra-assays of RF was 1.83%

and that of inter-assays of RF was 3.82%. The CV value of intra-assays of ASO was 2.76%

and that of inter-assays of ASO was 5.28%. Quantification detection has satisfactory line relation. (RF r =0.987，ASO r =0.995). The positive rate of ASO was 16.6% by nephelometry

while the positive rate of ASO was 8.3% by latex agglutination test. The positive rate of RF was 24.1% by nephelometry

while the positive rate of RF was 18.4% by latex agglutination test. Rate nephelometry has a higher positive rate compared with latex method. 25 normal samples were detected by this method

the mean value of RF was less than 20IU/ml

the mean value of ASO was 70.7±55.6IU/ml. Conclusion: This method is quite good in reproducibility

accuracy

stabilization

and specificity

and is not affected by lipid

hemolysis and jaundice. This method is valuable in clinical use.