The expression of p53 gene in peripheral blood lymphocyte of acute Kawasaki disease

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The expression of p53 gene in peripheral blood lymphocyte of acute Kawasaki disease

更新时间：2024-01-17

- The expression of p53 gene in peripheral blood lymphocyte of acute Kawasaki disease
- Journal of Chongqing Medical University Issue 1, Pages: 51-52(2001)
- 作者机构：
  
  1. 重庆医科大学儿科学院,重庆,400014
  2. 广东省深圳市儿童医院,深圳,518026
- 作者简介：
- 基金信息：
- DOI：
  CLC： R725.9
- Published：2001，
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[1]易岂建,杨锡强,李成荣,张远维,王莉佳.p53基因与川崎病患者淋巴细胞凋亡关系探讨[J].重庆医科大学学报,2001(01):51-52+55.

The expression of p53 gene in peripheral blood lymphocyte of acute Kawasaki disease. [J]. Journal of Chongqing Medical University (1):51-52(2001)
[1]易岂建,杨锡强,李成荣,张远维,王莉佳.p53基因与川崎病患者淋巴细胞凋亡关系探讨[J].重庆医科大学学报,2001(01):51-52+55. DOI：

The expression of p53 gene in peripheral blood lymphocyte of acute Kawasaki disease. [J]. Journal of Chongqing Medical University (1):51-52(2001) DOI：

摘要

目的：进一步探讨川崎病（KD）急性期患者外周血淋巴细胞凋亡延迟的机理。方法：采用斑点杂交（Dot-blot）检测淋巴细胞p53 基因mRNA表达水平；流式细胞仪（FCM）检测p53蛋白质表达阳性细胞百分率。结果： KD患者外周血淋巴细胞p53 基因mRNA和p53蛋白质表达水平降低，与正常儿童比较差异显著（Ｐ<0.005）；当给予静脉注射免疫球蛋白（IVIG）治疗后或加入抗IL-6单抗培养时，外周血淋巴细胞p53 基因mRNA和p53蛋白质表达水平提高。结论：KD急性期患者外周血淋巴细胞p53 基因表达水平降低，其原因可能与本病患者异常升高的IL-6有关。p53 基因具有促进细胞凋亡的作用，KD患者外周血淋巴细胞凋亡延迟可能与高浓度IL-6抑制p53 基因的表达有关。

Abstract

Objective: To further explore the mechanism of inhibited apoptosis of peripheral blood mononuclear cell (PBMC) in acute Kawasaki disease(KD). Methods: The expression level of p53 gene mRNA was determined by dot-blot; p53 protein positive cell percentage was detected by flow cytometry (FCM). Results: The expression of p53 gene mRNA and p53 protein in acute KD patients were decreased (Ｐ<0.001)

but increased after treating with intravenous immunoglobulin(IVIG) in vivo or adding anti-IL-6 monoantibody(mAb) into PBMC culture in vitro. Conclusion: The decreased expression of p53 gene mRNA and p53 protein may be associated with the high concentration of IL-6 in KD patients. p53 gene expression could induce lymphocyte apoptosis. Thus

the expression of p53 gene inhibited by the increased IL-6 production might be related to delaying or depressing apoptosis of PBMC in KD.

关键词

川崎病细胞凋亡p53基因白细胞介素-6

Keywords

Kawasaki diseaseapoptosisp53 geneinterleukin-6

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