Clinicopathologic value of detection of  esophageal  squamous carcinoma micrometastases in regional lymph nodes by means of reverse transcriptase-polymerase chain reaction

GE Mingjian; et al

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Clinicopathologic value of detection of esophageal squamous carcinoma micrometastases in regional lymph nodes by means of reverse transcriptase-polymerase chain reaction

更新时间：2024-01-17

- Clinicopathologic value of detection of esophageal squamous carcinoma micrometastases in regional lymph nodes by means of reverse transcriptase-polymerase chain reaction
- Journal of Chongqing Medical University Issue 4, Pages: 417-419(2002)
- 作者机构：
  
  1. 重庆医科大学临床学院胸心外科
  2. ,重庆医科大学临床学院胸心外科
  3. 重庆医科大学临床学院检验科,重庆医科大学临床学院检验科重庆　400016,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.1
- Published：2002，
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[1]葛明建,李良彬,王梅,张玉洪.食管鳞癌区域淋巴结微转移分子学检测的临床病理意义[J].重庆医科大学学报,2002(04):417-419.

GE Mingjian, et al. Clinicopathologic value of detection of esophageal squamous carcinoma micrometastases in regional lymph nodes by means of reverse transcriptase-polymerase chain reaction. [J]. Journal of Chongqing Medical University (4):417-419(2002)
[1]葛明建,李良彬,王梅,张玉洪.食管鳞癌区域淋巴结微转移分子学检测的临床病理意义[J].重庆医科大学学报,2002(04):417-419. DOI：

GE Mingjian, et al. Clinicopathologic value of detection of esophageal squamous carcinoma micrometastases in regional lymph nodes by means of reverse transcriptase-polymerase chain reaction. [J]. Journal of Chongqing Medical University (4):417-419(2002) DOI：

摘要

目的：评价RT-PCR法检测食管鳞癌区域淋巴结微转移的临床病理意义。方法：取23例食管鳞癌患者的区域淋巴结共104枚，将每枚淋巴结均分为两等份，分别进行病理学检查和上皮组织特异性标志物角蛋白CK19基因的表达分析。对两法测得的淋巴结转移结果与常见临床病理参数间的关系进行统计学分析。结果：23例病人中，病理检查发现其中10例（43.5%）淋巴结存在癌转移，淋巴结是否转移与临床病理参数之间不存在相关性。CK19RT-PCR法不但证实了病理检查的结果，而且还另外发现5例病人存在淋巴结微转移，淋巴结转移与否与组织分化等级（P =0.035）和pTNM分期（P =0.024）间存在相关关系。结论：运用RT-PCR法检测区域淋巴结转移有助于揭示食管癌固有的转移规律。

Abstract

Objective: To evaluate the clinicopathologic value of means of reverse transcriptase-polymerase chain reaction (RT-PCR) which could be used to detect micrometastases in regional lymph nodes(LNs) of patients with esophageal sequamous carcinoma (ESC). Methods: The regional LNs( n =104) were obtained from 23 ESC patients who underwent operation. Each LN was cut into halves. One half of each LN was subjected to histological examination (HE). The other half was subjected to CK19RT-PCR.The correlative relationship of results of metastases in regional LNs and common clinicopathological parameters were analyzed statisticly. Results: Among 23 patients analyzed in this study

histological examination was done on each LN

and it revealed metastases in LNs of 10 patients. At the same time these results were confirmed by CK19RT-PCR.Of the 13 patients who were diagnosed to be devoid of metastases by HE，five patients were found to exist micrometastases in regional LNs.The detective rate of metastases in regional LNs was evaluated from 43.5% by HE to 65.2% by CK19RT-PCR.As far as the results of metastases in regional LNs in 23 ESC patients by CK19RT-PCR were concerned

the presence of micrometastases in LNs was associated with grade (P =0.035

exact test) as well as pTNM stage (P =0.024

exact test). Conclusion:The assay of RT-PCR which is used to detect micrometastases in regional LNs of ESC patients is helpful for disclosing the inherent metastatic role of this disease.

关键词

食管肿瘤淋巴转移诊断角蛋白聚合酶链反应

Keywords

Esophageal neoplasmsLymphatic metastasisdiagnosisKeratinPolymerase chain reaction

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葛明建

李良彬

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Related Institution

Department of Radiology，The First Affiliated Hospital of Chongqing Medical University

Teaching and Research Section of Pathology，College of Basic Medical Sciences，Chongqing Medical University

Department of Traditional Chinese Medicine，Children’s Hospital，Zhejiang University School of Medicine/ National Clinical Research Center for Child Health

Department of Pediatrics，The Affiliated Hospital of Nanjing University of Chinese Medicine，The First Clinical Medical College of Nanjing University of Chinese Medicine

Department of Respiratory Medicine，Children’s Hospital，Zhejiang University School of Medicine/ National Clinical Research Center for Child Health

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