Detection of plasmid-mediated AmpC expressed in Escherichia coli and Klebsiella pneumoniae by cefoxitin three-dimensional test

XIA Yun; et al

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Detection of plasmid-mediated AmpC expressed in Escherichia coli and Klebsiella pneumoniae by cefoxitin three-dimensional test

更新时间：2024-01-17

- Detection of plasmid-mediated AmpC expressed in Escherichia coli and Klebsiella pneumoniae by cefoxitin three-dimensional test
- Journal of Chongqing Medical University Issue 4, Pages: 424-426(2002)
- 作者机构：
  
  1. 重庆医科大学临床学院检验科
  2. 重庆医科大学临床学院检验科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.5
- Published：2002，
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[1]夏云,赵世巧.用头孢西丁三相试验检测大肠埃希菌及肺炎克雷伯菌的质粒AmpC酶[J].重庆医科大学学报,2002(04):424-426.

XIA Yun, et al. Detection of plasmid-mediated AmpC expressed in Escherichia coli and Klebsiella pneumoniae by cefoxitin three-dimensional test. [J]. Journal of Chongqing Medical University (4):424-426(2002)
[1]夏云,赵世巧.用头孢西丁三相试验检测大肠埃希菌及肺炎克雷伯菌的质粒AmpC酶[J].重庆医科大学学报,2002(04):424-426. DOI：

XIA Yun, et al. Detection of plasmid-mediated AmpC expressed in Escherichia coli and Klebsiella pneumoniae by cefoxitin three-dimensional test. [J]. Journal of Chongqing Medical University (4):424-426(2002) DOI：

摘要

目的：检测质粒介导的持续高产AmpC酶在大肠埃希菌及肺炎克雷伯菌中的携带率，为临床治疗提供指导。方法：采用头孢西丁纸片药敏试验（K-B法）作AmpC酶的初筛试验，头孢西丁三相试验间接法作AmpC酶的确证试验。结果：在检测的86株菌中，有9株菌K-B法初筛结果为阳性。在这9株菌中经头孢西丁三相试验确证有5株产AmpC酶，阳性率为5.8%。其中大肠埃希菌3株，肺炎克雷伯菌2株。结论：头孢西丁K-B法筛选试验结合头孢西丁三相试验可准确检出质粒介导的AmpC酶，可用于临床常规检测。

Abstract

Objective: To detect the prevalence rate of the plasmid-mediated AmpC expressed constitutively at high level in Escherichia coli and Klebsiella pneumoniae for providing guideline for clinical therapy. Methods: Cefoxitin susceptibility disk test (K-B method) was used for screening and indirect cefoxitin three-dimensional test was used for the confirming of plasmid-mediated AmpC. Results: Of 86 strains

9 isolates were positive by K-B screening method. Of the 9 positive isolates

5 (three Escherichia coli

two Klebsiella pneumoniae) were positive by indirect cefoxitin three-dimensional confirming test

with a positive rate of 6.9%. Conclusion:Cefoxitin screening test combinated with indirect three-dimensional test can detect plasmid-mediated AmpC accurately and can be used in clinical routine detection.

关键词

三相试验质粒AmpC酶大肠埃希菌肺炎克雷伯菌

Keywords

Three-dimensional testPlasmid-mediated AmpCEscherichia coliKlebsiella pneumoniae

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