Inhibitory effects of TIMP-3 on endothelial cell proliferation

LI Shengwei; et al

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Inhibitory effects of TIMP-3 on endothelial cell proliferation

更新时间：2024-01-17

- Inhibitory effects of TIMP-3 on endothelial cell proliferation
- Journal of Chongqing Medical University Issue 1, Pages: 26-30(2006)
- 作者机构：
  
  1. 重庆医科大学附属第二医院肝胆外科
  2. 重庆市肿瘤医院肿瘤研究所
  3. 重庆医科大学检验系
  4. 重庆医科大学附属第二医院肝胆外科,重庆,400010
  5. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R363
- Published：2006，
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[1]李生伟,甘霖,王一如,刘长安,龚建平,李钺,李友伟.TIMP-3对内皮细胞增殖抑制作用的研究[J].重庆医科大学学报,2006(01):26-30+34.

LI Shengwei, et al. Inhibitory effects of TIMP-3 on endothelial cell proliferation. [J]. Journal of Chongqing Medical University (1):26-30(2006)
[1]李生伟,甘霖,王一如,刘长安,龚建平,李钺,李友伟.TIMP-3对内皮细胞增殖抑制作用的研究[J].重庆医科大学学报,2006(01):26-30+34. DOI：

LI Shengwei, et al. Inhibitory effects of TIMP-3 on endothelial cell proliferation. [J]. Journal of Chongqing Medical University (1):26-30(2006) DOI：

摘要

目的:探讨TIM P-3对内皮细胞增殖的抑制作用及特点。方法:采用形态学观察、M TT法、[3H]-TdR掺入法流式细胞仪分析等技术方法观测不同浓度TIM P-3作用下人脐静脉内皮细胞增殖活性的变化。结果:①不同浓度（10～100ng/m l）的TIM P-3处理H UVEC细胞不同时间（1～7天）后

生长均有显著抑制作用且呈现明显的剂量和时效依赖关系。②经TIM P-3处理后

H UVEC细胞增殖活性降低

主要表现为有丝分裂计数降低

细胞的生长抑制率及移行抑制率增加。③细胞生长减慢

TIM P-3能有效抑制G1、S期的细胞;抑制细胞从G2期进入M期;同时促进细胞的凋亡等。结论:TIM P-3具有抑制内皮细胞增殖、迁移、促进细胞凋亡

进而抑制血管生成的作用。

Abstract

Objective: To investigate the inhibitory effect of TIMP-3 on endothelial cell(EC) proliferation.Methods: The inhibition of EC proliferation was analyzed by light microscopy

MTT

-TdR up-take

and flow cytometry et al.Results: A concentration-dependent and time-dependent proliferation inhibition and survival were demonstrated in the HUVEC incubated for 1~7days in the presence of TIMP-3.The proliferation inhibition was shown as the lessened cell growth

decreased counting of mitotic cells.TIMP-3 could also aggravate the increase of apoptotic cells and cell ratio in G2/M phase.Conclusion: TIMP-3 has significant inhibitory influence on EC proliferation

migration and can promote apoptosis of cells.

关键词

TIMP-3内皮细胞细胞增殖细胞凋亡

Keywords

TIMP-3endothelial cellproliferationapoptosis

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