Objective:To investigate the profile of the distribution and drug resistances of clinic pathogenic microorganism isolated in 2005.Methods:The microorganisms were isolated from clinical samples according to the guides of standard operation protocol.Antibiotic sensitivity tests were done by the method of micro broth dilution tests and the values of the MIC were compared by the guides of American Clinical Laboratory Standard Institution.Results:In total 2352 isolated strains

most of them were Gram negative bacilli and mainly caused respiratory tract infection.The isolating rates for Candida spp and Enterococcus spp have increased dramatically compared with the results of 2004.The ten pathogens ranking top isolating rates were Escherichia coli

Pseudomonas aeruginosa

Candida albicans

Acinetobacter baumannii

Klebsiella pneumoniae

Enterococcus faecalis

Candida tropicalis

Enterobacter cloacae

Staphylococcus aureus

Staphylococcus haemolyticus respectively.The antibiotics exhibiting high activity against common gram-negative bacilli such as Escherichia coli

Pseudomonas aeruginosa

Klebsiella pneumoniae

Acinetobacter baumannii and Pseudomonas aeruginosa were imipenem

meraopenem

amikacin and piperacillin/tazobactam.Vancomycin

teicoplanin and nitrofurantoin remained high activity against Enterococcus spp and Staphylococcus spp.Candida albicans and Candida tropicalis were susceptible to amphotericin B

fluconazole

and itraconazole.Conclusion:The species of pathogens causing clinical infections and the profiles of their drug resistance have presented obvious variation

especially the pathogens that cause nosocomial infections exhibit high degree of resistance to the antibiotics that conventionally used in clinics.The surveillance of drug resistance is important in the guiding practice of clinical infection control.