Objective: To evaluate the microbial safety of human amniotic membrane(HAM) in preparation and preservation

and to establish effective methods of microbe control for HAM bank.Methods: Human amnions were collected from elective caesarean sections in 20 healthy women

and divided into groups based on different sterilization procedures and preservation methods.Bacteria

fungi and mycoplasmas were detected at 24h

1m

3m and 12m after preservation.Other amnions collected in eutocia from 5 healthy women were detected bacteria

fungi

lactobacilli and mycoplasmas.The series of serological determination for the parturients were followed up in 6 months.Results: Two HAM samples only soaked in NS were detected to be contaminated with E.coli and/or S.epidermidis

respectively from two caesarean sections.There is no microbe detected in all of the amnions from caesarean sections which were soaked in the antibiotics solution.One HAM sample from eutocia was yet detected to have lactobacilli after soak.The series of serological determination were all negative.Conclusion: During one year storage

HAM only from caesarean sections in healthy women

can successfully avoid contamination if soaked in the antibiotics solution then prepared and preserved with aseptic technique.