The study of killing effect by T-lymphocytes after activiated by dendritic cells loaded with RCC 786-0 cell line freeze thawing antigen in vitro

HU Zili; et al

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The study of killing effect by T-lymphocytes after activiated by dendritic cells loaded with RCC 786-0 cell line freeze thawing antigen in vitro

更新时间：2024-01-17

- The study of killing effect by T-lymphocytes after activiated by dendritic cells loaded with RCC 786-0 cell line freeze thawing antigen in vitro
- Journal of Chongqing Medical University Issue 4, Pages: 513-517(2006)
- 作者机构：
  
  1. 重庆医科大学附属第二医院泌尿外科
  2. 重庆医科大学附属第二医院泌尿外科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.11
- Published：2006，
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[1]胡自力,麦选诚.肾癌冻融抗原致敏树突状细胞介导的CTL对肾癌786-0细胞株的体外杀伤作用研究[J].重庆医科大学学报,2006(04):513-517+551.

HU Zili, et al. The study of killing effect by T-lymphocytes after activiated by dendritic cells loaded with RCC 786-0 cell line freeze thawing antigen in vitro. [J]. Journal of Chongqing Medical University (4):513-517(2006)
[1]胡自力,麦选诚.肾癌冻融抗原致敏树突状细胞介导的CTL对肾癌786-0细胞株的体外杀伤作用研究[J].重庆医科大学学报,2006(04):513-517+551. DOI：

HU Zili, et al. The study of killing effect by T-lymphocytes after activiated by dendritic cells loaded with RCC 786-0 cell line freeze thawing antigen in vitro. [J]. Journal of Chongqing Medical University (4):513-517(2006) DOI：

摘要

目的:研究肾癌冻融抗原负载树突状细胞（DCs）诱导的特异性细胞毒性T淋巴细胞（CTL）对肾癌786-0细胞株的体外杀伤效应;方法:利用人外周血体外经重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）、人白介素-4（rhIL-4）和肿瘤坏死因子（TNF-α）诱导产生的DCs负载肾癌冻融裂解物后诱导产生特异性CTL

观察杀伤后肿瘤细胞的形态变化

MTT法测定细胞毒性试验

ELISA测定细胞因子的分泌。结果:（1）负载肾癌抗原的DCs能促进T细胞的增殖;（2）诱导产生的特异性CTL对肾癌细胞具有高杀伤率

显著高于非特异性T细胞（P<0.05）;（3）肾癌冻融抗原能够上调DCs人白介素-12（rhIL-12）的分泌

提高抗肿瘤的作用。（4）杀伤后的肾癌细胞发生明显凋亡。结论:由肾癌冻融抗原致敏的DCs所诱导产生的抗原特异性CTL对肾癌786-0细胞株有明显的杀伤效应

提示DCs瘤苗具有为肾癌患者进行特异性过继免疫治疗的临床应用前景。

Abstract

Objective: To study the immune responses of cytotoxic T-lymphocytes against Renal carcinoma cell 786-0(RCC) after activiated by dendritic cells(DCs) loaded with RCC freeze thawing antigen.Methods:DCs were obtained by cultured plastic-adherent monocytes isolated from health human peripheral blood with granulocyte-monocyte colony-stimulating factor(GM-CSF) and interleukin-4(IL-4) and Tumor necrosis factor(TNF) for 9 days.Host lymphocytes were stimulated with freeze thawing antigen of RCC 786-0 under the culture medium containing interleukin-2(IL-2) for 5 day.Killing activity and cytokine release were measured by MTT assay and ELISA.Results:The immune response of CTL activiated by DCs loaded with tumor soluble antigen was demonstrated by the following facts:(1)DCs loaded with tumor antigen could induce the growth of CTL.(2)The cyotoxicity of obtained specific CTL against RCC-7860 was highly enhanced with a significant difference from that of non-specific CTL.(3)The interleukin-12 release/secretion was increased by tumor antigen

which suggest an improved anti-tumor effect.(4)Apoptosis was observed in the RCCs after treated with CTL obtained.Conclution: These findings indicate that specific CTL induced by DCs sensibilized by RCC freeze thawing antigen exerts a remarkable killing activity on RCC 786-0.It is suggestd that DCs antitumor vaccines poses a clinically useful prospect with RCC.

关键词

肾细胞癌树突状细胞抗肿瘤免疫CTL

Keywords

Renal carcinoma celldendritic cellsAntitumor immuneCTL

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