Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence

DENG Jianchuan; et al

您当前的位置：

首页 >

文章列表页 >

Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence

更新时间：2024-01-17

- Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence
- Journal of Chongqing Medical University Issue 4, Pages: 463-465(2006)
- 作者机构：
  
  1. 重庆医科大学附属第二医院病毒性肝炎研究所
  2. 重庆医科大学附属第二医院病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R346
- Published：2006，
扫描看全文
[1]邓建川,刘杞,陈曜,孙航,唐琳,王娜.利用5′-RACE技术进行人肝再生增强因子相互作用肽cDNA5′末端快速扩增及序列分析[J].重庆医科大学学报,2006(04):463-465.

DENG Jianchuan, et al. Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence. [J]. Journal of Chongqing Medical University (4):463-465(2006)
[1]邓建川,刘杞,陈曜,孙航,唐琳,王娜.利用5′-RACE技术进行人肝再生增强因子相互作用肽cDNA5′末端快速扩增及序列分析[J].重庆医科大学学报,2006(04):463-465. DOI：

DENG Jianchuan, et al. Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence. [J]. Journal of Chongqing Medical University (4):463-465(2006) DOI：

摘要

目的:利用5′-RACE技术进行人肝再生增强因子（hALR）相互作用肽cDNA5′末端快速扩增并进行序列分析。方法:按照5′-RACE试剂盒（Takara）操作流程进行hALR相互作用肽cDNA5′末端快速扩增

用BLAST技术对扩增序列进行生物信息学分析。结果:成功获取487bp的5′-RACE反应产物

其中5′端延伸了275bp

同源序列分析表明与人Citron激酶mRNA高度同源。结论:5′-RACE技术可以用于快速有效扩增已知部分cDNA序列的5′末端

Citron激酶可能是与hALR具有相互作用的蛋白。

Abstract

Objective: To amplify the 5′ cDNA end of human augmenter of liver regeneration(hALR) by 5′-RACE technique and to analyze the sequence.Methods: 5′ cDNA end of hALR was rapidly amplified by following the instructions of 5′-RACE kit(Takara).The acquired sequence was analyzed by bioinformatic tool.Results: 5′-RACE product with 487bp was successfully obtained

which included extending sequence with 275bp.Homology analysis by BLAST showed highly homologous with human citron kinase.Conclusions: 5′-RACE method can be used to amplify the 5′ end of partially known cDNA sequence.Citron kinase can interact with hALR.

关键词

5’-RACE人肝再生增强因子相互作用肽cDNA

Keywords

5’-RACEhALRInteracting peptidecDNA

references

Views

360

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰