Research on DNA methylation in 5′CpG island of P16 gene and P16 expression in primary breast cancer and benign breast lesions

ZHOU Linyan; et al

您当前的位置：

首页 >

文章列表页 >

Research on DNA methylation in 5′CpG island of P16 gene and P16 expression in primary breast cancer and benign breast lesions

更新时间：2024-01-17

- Research on DNA methylation in 5′CpG island of P16 gene and P16 expression in primary breast cancer and benign breast lesions
- Journal of Chongqing Medical University Issue 2, Pages: 175-179(2006)
- 作者机构：
  
  1. 重庆医科大学病理学教研室
  2. 重庆医科大学病理学教研室重庆400016 常州市肿瘤医院病理科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2006，
扫描看全文
[1]周林艳,张徽,李圆圆,林晓.乳腺癌及良性病变中P16蛋白与基因5′CpG岛甲基化的病理意义的探讨[J].重庆医科大学学报,2006(02):175-179.

ZHOU Linyan, et al. Research on DNA methylation in 5′CpG island of P16 gene and P16 expression in primary breast cancer and benign breast lesions. [J]. Journal of Chongqing Medical University (2):175-179(2006)
[1]周林艳,张徽,李圆圆,林晓.乳腺癌及良性病变中P16蛋白与基因5′CpG岛甲基化的病理意义的探讨[J].重庆医科大学学报,2006(02):175-179. DOI：

ZHOU Linyan, et al. Research on DNA methylation in 5′CpG island of P16 gene and P16 expression in primary breast cancer and benign breast lesions. [J]. Journal of Chongqing Medical University (2):175-179(2006) DOI：

摘要

目的:探讨P16蛋白在乳腺良性病变

乳腺导管内癌

浸润性导管癌中的表达

p16基因5′CpG岛甲基化在原发性乳腺癌组织中与P16蛋白失表达的关系及意义。方法:用限制性酶PCR检测45例乳腺浸润性导管癌及15例乳腺良性病变p16基因中5′CpG岛甲基化状态

甲基化检测结果与免疫组织化学方法检测的P16蛋白表达结果进行比较。结果:p16蛋白在乳腺癌中强表达

但随组织学分级升高

淋巴结的转移

肿瘤直径的增加

p16蛋白表达率降低。p16基因甲基化与p16蛋白表达负相关

且与组织学分级相关。结论:p16基因甲基化是其失表达的可能机制之一。

Abstract

Objective: To examine the involvement of methylation of p16 gene on the p16 status and tumor progression in primary breast cancer and the role of p16 protein in primary breast cancer.Methods: DNA was extracted from 45 cases of primary breast carcinomas and 15 cases of benign breast lesions.Polymerase chain reaction(PCR)

using the extracted DNA and methylation-sensitive restriction enzymes(HpaⅡ?SacⅡ)

was performed to detect the methylation of 5′CpG island in the p16 gene.The results were compared with the P16 expression by immunostaining.Results:(1) p16 was overexpressed in breast cancer.With the increase of histological grade of breast carcinomas

p16 expression decreased.In addition

the low expression of p16 in primary breast cancer was associated with tumor size and lymph node involvement.(2) Methylation of the CpG island of the p16 gene was inversely correlated with p16 expression in a subset of the primary breast carcinomas.With the increase of histological grade of breast carcinomas

DNA methylation was enhanced.Conclusion:Methylation of p16 gene may be the cause of loss of p16 expression.

关键词

DNA甲基化乳腺肿瘤CpG岛P16

Keywords

DNA methylationBreast cancerCpG islandP16

references

Views

114

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Value of abnormal methylation of cg08721802 locus in differential diagnosis of benign and malignant pulmonary nodules

Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia

Study on the effect of AR, cyclinD1, p16 in laryngeal squamous cell carcinoma and their interaction

P16 and cyclinD₁ expression in carcinogenesis of gastric mucosa

Expression of VEGF-C in human breast cancer and its implication

Related Author

Peilong Li

Yan Xie

Lutao Du

Chuanxin Wang

Suhong Ma

Qi Hongbo

Ding Yubin

Shan Nan

Related Institution

Department of Clinical Laboratory，The Second Hospital of Shandong University/Tumor Marker Detection Engineering Laboratory of Shandong Province

Laboratory of Reproductive Biology,School of Public Health and Management,Chongqing Medical University,Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine of Chongqing Medical University

Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University,Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine of Chongqing Medical University

重庆医科大学第二临床学院耳鼻咽喉科

Department of Otorhinolaryngology, Second College of Clinical Medicine, Chongqing Medical University

⁰