The study of green tea polyphenols inhibiting apoptosis of lens epithelial cell in vitro and relevant mechanism

ZHOU Xi; et al

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The study of green tea polyphenols inhibiting apoptosis of lens epithelial cell in vitro and relevant mechanism

更新时间：2024-01-17

- The study of green tea polyphenols inhibiting apoptosis of lens epithelial cell in vitro and relevant mechanism
- Journal of Chongqing Medical University Issue 2, Pages: 190-192(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院眼科
  2. 重庆医科大学附属第一医院眼科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R776.1
- Published：2006，
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[1]周晞,李平华.茶多酚抗晶状体上皮细胞凋亡的体外研究及机制[J].重庆医科大学学报,2006(02):190-192+203.

ZHOU Xi, et al. The study of green tea polyphenols inhibiting apoptosis of lens epithelial cell in vitro and relevant mechanism. [J]. Journal of Chongqing Medical University (2):190-192(2006)
[1]周晞,李平华.茶多酚抗晶状体上皮细胞凋亡的体外研究及机制[J].重庆医科大学学报,2006(02):190-192+203. DOI：

ZHOU Xi, et al. The study of green tea polyphenols inhibiting apoptosis of lens epithelial cell in vitro and relevant mechanism. [J]. Journal of Chongqing Medical University (2):190-192(2006) DOI：

摘要

目的:探讨抗氧化剂茶多酚对氧化损伤所诱发的晶状体上皮细胞凋亡的抑制作用和相关机制。方法:分离培养兔晶状体上皮细胞

茶多酚处理24～72h后

采用透射电镜、流式细胞术、凋亡细胞DNA片断分析检测茶多酚抗晶状体上皮细胞凋亡的作用

并用Western-blot检测凋亡相关蛋白bcl-2、Bax、caspase-3蛋白表达变化

揭示茶多酚抑制晶状体上皮细胞凋亡的相关机制。结果:氧化损伤型晶状体上皮细胞生理盐水处理组中

凋亡细胞增多

G1期细胞升高

DNA片断分析呈现典型的凋亡细胞特征性“梯状条带”;茶多酚处理组中

凋亡细胞明显减少

G1期细胞降低

未出现特征性“梯状条带”

凋亡相关蛋白bcl-2增多、Bax降低、caspase-3蛋白减少。结论:抗氧化剂茶多酚可抑制H2O2氧化损伤所诱导的兔晶状体上皮细胞凋亡并减轻或延缓白内障形成

其机制与bcl-2、Bax及细胞凋亡过程中最重要的终末执行酶caspase-3蛋白表达变化密切相关。

Abstract

Objective: To investigate the inhibitory effect of Green Tea Polyphenols(GTP) on H2O2-induced lens epithelial cell apoptosis and relevant mechanism.Methods: Separated and cultivated lens epithelial cell

disposed that with GTP

then detected the inhibitory effect of GTP by electron transmission microscopy

flow cytometry and DNA fragmentation assay.The bcl-2

Bax and caspase-3 protein expression were measured with Western-blot in various groups.Results: The apoptosis cells and G1 phase cells increased in the group treated with sodium chloride solution

and the DNA "ladder" was found.In the group treated with GTP

the apoptosis cells and G1 phase cells decreased

and no DNA "ladder".The apoptosis protein bcl-2 increased

however

the Bax and caspase-3 protein decreased.Conclusion: The GTP can protect lens epithelial cells from the oxidative injury and restrain the apoptosis rate of lens epithelial cells and the mechanism was nearly correlated with bcl-2

Bax and caspase-3 protein.

关键词

茶多酚晶状体上皮细胞氧化损伤凋亡机制

Keywords

Green Tea PolyphenolsLens epithelial cellOxidative injuryapoptosismechanism

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