Comparison of colony-forming units and drug susceptibility to mycobacteria between weighting method and Mcfarland method

LI Youlun; et al

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Comparison of colony-forming units and drug susceptibility to mycobacteria between weighting method and Mcfarland method

更新时间：2024-01-17

- Comparison of colony-forming units and drug susceptibility to mycobacteria between weighting method and Mcfarland method
- Journal of Chongqing Medical University Issue 1, Pages: 88-90(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肺科
  2. 中国药品生物制品检定所
  4. ,北京,100050
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.5
- Published：2006，
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[1]黎友伦,陈保文,沈小兵,彭丽,王国治.比较称重法和比浊法对分枝杆菌活菌计数和药敏的差异[J].重庆医科大学学报,2006(01):88-90+131.

LI Youlun, et al. Comparison of colony-forming units and drug susceptibility to mycobacteria between weighting method and Mcfarland method. [J]. Journal of Chongqing Medical University (1):88-90(2006)
[1]黎友伦,陈保文,沈小兵,彭丽,王国治.比较称重法和比浊法对分枝杆菌活菌计数和药敏的差异[J].重庆医科大学学报,2006(01):88-90+131. DOI：

LI Youlun, et al. Comparison of colony-forming units and drug susceptibility to mycobacteria between weighting method and Mcfarland method. [J]. Journal of Chongqing Medical University (1):88-90(2006) DOI：

摘要

目的:比较用称重法和比浊法对分枝杆菌活菌计数、对结核分枝杆菌（M TB）绝对浓度法和比例法药物敏感性（药敏）结果的差异。方法:用称重法和比浊法制作非结核分枝杆菌（NTM）28株、M TB42株菌悬液

10倍梯度稀释

10-4m g/m l、10-5/m g/m l、10-6m g/m l菌悬液0.1m l接种于改良L-J培养基上作活菌计数

10-2m g/m l菌悬液0.1m l接种于含药改良L-J培养基上作绝对浓度法药敏

10-3m g/m l、10-5m g/m l菌悬液0.1m l接种于含药改良L-J培养基上作比例法药敏。结果:两种方法NTM活菌计数比较有显著性差异（P<0.05）

活菌计数值在2×107～8×108CFU/m l;两种方法M TB活菌计数值比较无显著性差异（P>0.05）

在5×106～5×107CFU/m l。M TB称重法和比浊法菌悬液绝对浓度法药敏一致率异烟肼、利福平分别达95.2%、97.6%

与比例法比较符合率异烟肼、利福平分别达95.2%、92.8%。结论:分枝杆菌不同菌株两种方法活菌计数值有较大差异

药敏结果一致性好。

Abstract

Objective: To compare the difference in colony-forming units(CFU) of mycobacteria and drug susceptibility to mycobacterium tuberculosis(MTB) between weighting method and Mcfarland method.Methods: The bacterial suspensions of non-tuberculosis mycobacteria(NTM) 28 strains

MTB 42 strains were prepared by using both weighting method and Mcfarland method.0.1ml of bacterial suspensions(10-4mg/ml

10-5mg/ml

10-6mg/ml) were incubated for CFU of mycobacteria.0.1ml of bacterial suspensions(10-2mg/ml) of MTB were incubated for the drug susceptibility testing of absolute concentration method.0.1ml of bacterial suspensions(10-3mg/ml

10-5mg/ml) of MTB were incubated for the drug susceptibility testing of proportion method.Results: The CFU of NTM showed significant difference(P<0.05)

the CFU quantity of NTM was from 2×107 CFU/ml to 8×108CFU/ml in weighting method and Mcfarland method.The CFU of MTB did not show significant difference(P>0.05)

the CFU quantity of MTB was from 5×106CFU/ml to 5×107CFU/ml in weighting method and Mcfarland method.The accordance rate of drug susceptibility of MTB with absolute concentration method between weighting method and Mcfarland method were 95.2% and 97.6% in INH and RFP

respectively.The accordance rate of drug susceptibility of MTB were 95.2% and 92.8% in absolute concentration method and proportion method about Isonizid and Rifampicin

respectively.Conclusion: The CFU of different mycobacteria strains are significant different.The accordance rate of drug susceptibility of MTB between weighting method and Mcfarland method is high.

关键词

分枝杆菌活菌计数药敏试验

Keywords

mycobacteriumColony-forming unitsDrug susceptibility testing

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