Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV<sub>3</sub> cell lines

WU Yongzhong; et al

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Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV₃ cell lines

更新时间：2024-01-17

- Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV₃ cell lines
- Journal of Chongqing Medical University Issue 1, Pages: 19-22(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肿瘤科
  2. 重庆医科大学基础医学院核医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.31
- Published：2006，
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[1]吴永忠,任庆兰,李少林.c-af-1反义寡核苷酸转染对人卵巢癌SKOV_3细胞增殖的抑制作用[J].重庆医科大学学报,2006(01):19-22+41.

WU Yongzhong, et al. Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV₃ cell lines. [J]. Journal of Chongqing Medical University (1):19-22(2006)
[1]吴永忠,任庆兰,李少林.c-af-1反义寡核苷酸转染对人卵巢癌SKOV_3细胞增殖的抑制作用[J].重庆医科大学学报,2006(01):19-22+41. DOI：

WU Yongzhong, et al. Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV₃ cell lines. [J]. Journal of Chongqing Medical University (1):19-22(2006) DOI：

摘要

目的:探讨c-raf-1基因反义寡核苷酸转染对人卵巢癌SKOV3细胞增殖的抑制作用及其分子机制。方法:实验分3组:正常对照组、c-raf-1正义治疗组、c-raf-1反义治疗组。脂质体转染后不同时间分别进行M TT试验、集落形成试验、荧光显微镜检测、蛋白质荧光强度测定

以观察不同条件处理后各组细胞的增殖、凋亡、蛋白质表达有无差别。结果:反义治疗组与正义治疗组比较:转染72h OD值分别0.272与1.307（P<0.05）;克隆形成率分别为30.6%与75.0%（P<0.05）;凋亡率分别为19.7%与9.2%（P<0.05）

同时明显地下调P74蛋白质的表达水平（P<0.05）。结论:c-raf-1反义寡核苷酸对人卵巢癌SKOV3细胞的增殖有明显的抑制作用。

Abstract

Objective: To investigate the effects and the mechanisms of c-raf-1 genes antisense oligodeoxynucleotides(ASODN) transfection in inhibiting the human ovarian carcinoma SKOV3 cell lines.Methods: There were 3 groups in our study: normal control group

c-raf-1 sense oligodeoxynucleotides(SODN) experimental group

and c-raf-1 antisense experimental group.at the different time points after liposome-mediated transfection

the cell proliferation

apoptosis

protein expressing level were observed by MTT assay

flow cytometry

fluorescent microscope and cloning test.Results: In the ASODN experimental group and SODN group

the OD-value were 0.272 and 1.307 respectively(P<0.05) 72h after transfection;the cloning efficiency were 30.6% and 75.0% respectively(P<0.05);the apoptosis rate were 19.7% and 9.2% respectively(P<0.05) and the level of P74 protein was decreased statistically in c-raf-1 ASODN group.Conclusions: The proliferation of the human ovarian carcinoma SKOV3 cell lines can be inhibited by the c-raf-1 ASODN.

关键词

SKOV3细胞反义寡核苷酸卵巢癌c-raf-1

Keywords

SKOV3 cell linesAntisense oligodeoxynucleotidesovarian carcinomac-raf-1

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Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV3 cell lines

DOI：

摘要

Abstract

关键词

Keywords

references

Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV₃ cell lines