Expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro

LUO Hongxia; et al

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Expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro

更新时间：2024-01-17

- Expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro
- Journal of Chongqing Medical University Issue 3, Pages: 322-325(2006)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理生理教研室
  2. 重庆医科大学基础医学院病理生理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2006，
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[1]罗红霞,邓华瑜,陈黎.HSP90在乳腺癌细胞株ZR-75-30中的表达及对粘附侵袭能力的影响[J].重庆医科大学学报,2006(03):322-325.

LUO Hongxia, et al. Expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro. [J]. Journal of Chongqing Medical University (3):322-325(2006)
[1]罗红霞,邓华瑜,陈黎.HSP90在乳腺癌细胞株ZR-75-30中的表达及对粘附侵袭能力的影响[J].重庆医科大学学报,2006(03):322-325. DOI：

LUO Hongxia, et al. Expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro. [J]. Journal of Chongqing Medical University (3):322-325(2006) DOI：

摘要

目的:研究热休克蛋白（HeatShockProtein

HSP）90在乳腺癌细胞株ZR-75-30中的表达及对该细胞粘附侵袭能力的影响

并探讨相关机制及意义。方法:体外培养ZR-75-30乳腺癌细胞株

以HSP90抑制剂Geldanamycin（GA）作用处理;免疫印迹技术（Western-blot）检测GA处理前后HSP90在乳腺癌细胞株ZR-75-30中的表达情况;噻唑兰比色（MTT）法检测GA对ZR-75-30细胞的增殖抑制作用;噻唑兰比色（MTT）法检测细胞对人工基底膜（Matrigel）的粘附能力变化;TranswellCham-ber法观察癌细胞侵袭、迁移能力的改变。结果:HSP90抑制剂GA对ZR-75-30细胞具有增殖抑制作用

呈时间剂量依赖关系;GA作用后HSP90在乳腺癌细胞株ZR-75-30中表达明显下降;HSP90抑制剂GA处理的乳腺癌细胞ZR-75-30的粘附率较未加GA的对照组下降明显（P<0.01）;在GA未处理对照组侵袭、迁移实验穿膜细胞数分别为95.4±5.2

103±15.4与GA处理实验组穿膜细胞数46.2±4.9

52.4±8.4相比具有显著性差异（P<0.01）。结论:通过抑制HSP90在乳腺癌细胞株ZR-75-30中的表达

GA能明显减弱乳腺癌细胞株ZR-75-30增殖粘附侵袭能力

HSP90与乳腺癌细胞增殖侵袭转移能力相关。

Abstract

Objective:To investigate the expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro and the mechanism of anti-invasion.Methods:The protein expression level of HSP90 in ZR-75-30 before and after treatment with HSP90 inhibitor Geldanamycin was detected by Western-blotting;The effects of GA on the proliferation

invasion and motility of human breast cancer cell line ZR-75-30 in vitro were explored respectively by MTT colorimetric assay and transwell chamber.Results:After treatment with GA the protein expression level of HSP90 in ZR-75-30 was decreased

and the proliferation of ZR-75-30 cells was obviously inhibited in a dose and time-dependent manner.Compared with the control guoup

the adhesion

invasion and motility abilities of the ZR-75-30 cells were decreased significantly(P<0.01).Conclusion:The results showed that The proliferation and invasive ability of the human breast cancer cell line ZR-75-30 can be suppressed by Geldanamycin and heat shock protein(HSP)90 is associated with the invasive and metastatic abilities of breast cancer cell.

关键词

乳腺癌细胞HSP90Geldanamycin转移

Keywords

Breast cancerHSP90Geldanamycinmetastasis

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