A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism

YU Huarong; et al

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A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism

更新时间：2024-01-17

- A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism
- Journal of Chongqing Medical University Issue 1, Pages: 42-46(2006)
- 作者机构：
  
  1. 重庆医科大学基础医学院生理学教研室
  2. 重庆医科大学基础医学院生理学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.1
- Published：2006，
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[1]余华荣,张能,方海立,董毅龙.细胞因子诱导离体胰岛β细胞凋亡及其机理研究[J].重庆医科大学学报,2006(01):42-46.

YU Huarong, et al. A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism. [J]. Journal of Chongqing Medical University (1):42-46(2006)
[1]余华荣,张能,方海立,董毅龙.细胞因子诱导离体胰岛β细胞凋亡及其机理研究[J].重庆医科大学学报,2006(01):42-46. DOI：

YU Huarong, et al. A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism. [J]. Journal of Chongqing Medical University (1):42-46(2006) DOI：

摘要

目的:观察白细胞介素-1β（IL-1β）、肿瘤坏死因子（TNF）和γ-干扰素（IFN-γ）引起胰岛细胞凋亡、胰岛素分泌、bcl-xl和bax蛋白表达的变化。方法:应用体外单层培养的3￣5天的W istar大鼠胰岛细胞

分别检测IL-1β、TNF和IFN-γ对胰岛细胞凋亡细胞百分率、以及培养液中基础和高糖刺激下胰岛素分泌量、NO含量、NOS活性、bcl-xl和bax蛋白表达以及DNA片段的影响。结果:IL-1β、TNF和IFN-γ联合可诱导胰岛细胞凋亡率明显增加及DNA明显片段化

同时NO含量和NOS活性亦明显升高

胰岛素分泌明显降低

bcl-xl表达下降和bax表达增强（P<0.01）

且有时间和剂量依赖性。结论:细胞因子能诱导胰岛细胞凋亡

其机制可能与提高NOS活性从而增加NO的生成以及上调bax/bcl-xl比例有关。

Abstract

Objective: To investigate the apoptosis of isolated pancreatic islet cells and insulin secretion induced by combination of IL-1β

TNF and IFN-γ

and to investigate the mechanism of the change.Methods: Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro.NO production

NOS activity

insulin secretion

the protein expression of bcl-xl and bax

apoptotic rate of islet cells and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1β

TNF and IFN-γ were measured.Results: Combination of IL-1β

TNF and IFN-γ induced a significant increase in apoptotic percentage of pancreatic islet cells

NO production and NOS activity

as well as DNA ladder appearance

while a decrease in insulin content.Up-regulation of the protein expression of bax and down-regulation of the protein expression of bcl-xl were also induced(P<0.01).These effects occurred in a time and dose dependent manner.Conclusion: The cytokines can induce β cell apoptosis

the mechanism of which may be the activation of NOS and the increase of NO production and the upregulation of bax/bcl-xl proportion.

关键词

白细胞介素-1β细胞凋亡一氧化氮基因表达胰岛细胞

Keywords

interleukin-1βapoptosisnitric oxidegene expressionIslet cell

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