Effects of Genistein on proliferation of human hepatic cancer cell line SMMC-7721

ZHENG Weiping; et al

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Effects of Genistein on proliferation of human hepatic cancer cell line SMMC-7721

更新时间：2024-01-17

- Effects of Genistein on proliferation of human hepatic cancer cell line SMMC-7721
- Journal of Chongqing Medical University Issue 6, Pages: 836-840(2006)
- 作者机构：
  
  1. 重庆医科大学机能实验室
  2. 重庆医科大学病理生理学教研室
  3. 重庆医科大学中心实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2006，
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[1]郑维萍,汤为学,李龙江,赵华.Genistein对人肝癌细胞SMMC-7721的抗增殖作用[J].重庆医科大学学报,2006(06):836-840.

ZHENG Weiping, et al. Effects of Genistein on proliferation of human hepatic cancer cell line SMMC-7721. [J]. Journal of Chongqing Medical University (6):836-840(2006)
[1]郑维萍,汤为学,李龙江,赵华.Genistein对人肝癌细胞SMMC-7721的抗增殖作用[J].重庆医科大学学报,2006(06):836-840. DOI：

ZHENG Weiping, et al. Effects of Genistein on proliferation of human hepatic cancer cell line SMMC-7721. [J]. Journal of Chongqing Medical University (6):836-840(2006) DOI：

摘要

目的:探讨Genistein对人肝癌细胞SMMC-7721的抑制增殖作用及其机理。方法:采用MTT法测定Genistein在不同浓度、不同时间对SMMC-7721细胞抑制增殖的效应;流式细胞术分析细胞周期分布及凋亡率;透视电镜观察细胞超微结构改变;免疫细胞化学法检测细胞凋亡相关蛋白的表达。结果:Genistein可抑制SMMC-7721细胞的增殖

其抑制率与药物浓度和作用时间呈依赖关系;72h的IC50为29.0760μm ol/L;流式细胞仪分析证实Genistein能使SMMC-7721细胞聚积在G2/M期

凋亡率为8.81%（P<0.05）;透视电镜观察发现Genistein能诱导细胞凋亡;免疫细胞化学法显示:Bax、Fas、Myc、iNOS、NF-kB表达增加

Bcl-2、MTP53表达减低。结论:Genistein在体外可抑制SMMC-7721细胞增殖作用

是通过细胞阻滞在G2/M期和上调Bax、Fas、Myc、iNOS、NF-kB的表达及下调Bcl-2、MTP53表达诱导的细胞凋亡。

Abstract

Objective:To investigate the proliferation inhibitory effect of Genistein on human hepatic cancer cell line SMMC-7721.Method:The MTT method was used to assay the biologic activities of Genistein on SMMC-7721 at different times and in different doses.The cell cycle distribution and cell apoptotic rate were detected by flow cytometric analysis.The cell ultrastructure was observed by electron microscope.The apoptosis associated protein was detected with immunohistochemical technique.Result:Genistein could effectively inhibit SMMC-7721 in a dose and time dependent manner.IC50(72h) of Genistein to SMMC-7721 was 29.0760μmol/L.The cell growth was arrested at G2/M stage and the apoptotic rate was 8.81%.Genistein could lead to apoptosis

which was confirmed by electron microcope.The expression of Bax?Fas?Myc?iNOS?NF-kB were up-regulated

while the expression of Bcl-2

MTP53 were down-regulated.Conclusion: Genistein could inhibit the proliferation of SMMC-7721 in vitro

which is due to the arrest at G2/M stage and the apoptosis is related to the up-regulation of Bax

Fas

Myc

iNOS

NF-kB and down-regulation of Bcl-2

MTP53.

关键词

Genistein肝癌凋亡

Keywords

GenisteinHepatic-cancerapoptosis

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