Generation and characterization of fibre modified adenovirus vectors

QIU Feng; et al

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Generation and characterization of fibre modified adenovirus vectors

更新时间：2024-01-17

- Generation and characterization of fibre modified adenovirus vectors
- Journal of Chongqing Medical University Issue 3, Pages: 330-333(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院临床药理研究室
  2. 重庆医科大学附属第二医院核医学科
  3. 重庆医科大学附属第一医院临床药理研究室,重庆,400016
  4. ,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q782
- Published：2006，
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[1]邱峰,陈世知,陈虹.新型腺病毒载体的制备及其特性研究[J].重庆医科大学学报,2006(03):330-333.

QIU Feng, et al. Generation and characterization of fibre modified adenovirus vectors. [J]. Journal of Chongqing Medical University (3):330-333(2006)
[1]邱峰,陈世知,陈虹.新型腺病毒载体的制备及其特性研究[J].重庆医科大学学报,2006(03):330-333. DOI：

QIU Feng, et al. Generation and characterization of fibre modified adenovirus vectors. [J]. Journal of Chongqing Medical University (3):330-333(2006) DOI：

摘要

目的:本研究在普通腺病毒载体（Adv）纤毛头节区插入了具有整合素特异性的RGD肽

制备了一种新型RGD-Adv

以克服Adv基因转导的柯萨奇腺病毒受体依赖性。方法:用体外连接法构建RGD-Adv

并考察其基因转效率、稳定性及体外、体内毒性。结果:RGD-Adv可有效地实现向受试细胞转导基因

其转导能力随病毒量的增加而加强。结论:具有av亲和性的RGD-Adv是一种安全高效的新型腺病毒载体

将在基因治疗及实验研究中发挥重要作用。

Abstract

Objective: In the present study

We developed and assess an integrin-targeted fiber-mutant Ad vector containing the Arg-Gly-Asp(RGD) peptide motif on the fiber knob for the transfection of melanoma cell lines.Methods: We constructed RGD-Adv by in vitro ligation method

and investigated the efficiency of gene transdution in melanoma cells

studied its stability

in vitro

and in vivo toxicology.Results: For in vivo and in vitro

gene transfer the RGD-Adv more efficient than the vector containing wild type fiber via an CAR independent cell entry pathway.Conclusion: These results revealed that gene delivery by RGD-Adv was more efficient than that by conventional Adv and suggest that av-integrin-targeted Adv will be of great utility for gene therapy and gene transfer experiments.

关键词

腺病毒载体RGD肽整合素

Keywords

adenovirus vectorRGD peptidIntegrin

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Related Institution

重庆医科大学基础医学院生物化学教研室

芝加哥大学医学中心分子肿瘤研究室

Department of Biochemistry,College of Basic Medicine,Chongqing Medical University

重庆医科大学附属第一医院临床药理学研究室

Department of Clinical Pharmacology Lab,the First Affiliated Hospital,Chongqing Medical University

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