The mechanism of PTEN down-regulation on neuron intracellular free calcium and apoptosis

CHEN Lixue; et al

您当前的位置：

首页 >

文章列表页 >

The mechanism of PTEN down-regulation on neuron intracellular free calcium and apoptosis

更新时间：2024-01-17

- The mechanism of PTEN down-regulation on neuron intracellular free calcium and apoptosis
- Journal of Chongqing Medical University Issue 4, Pages: 505-508(2006)
- 作者机构：
  
  1. 重庆医科大学检验系临床生化室
  2. 第三军医大学大坪医院野战外科研究所三室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R743
- Published：2006，
扫描看全文
[1]陈力学,刘宝松,康格非,姜利人.PTEN表达下调对神经元胞内游离钙离子和神经元凋亡的研究[J].重庆医科大学学报,2006(04):505-508.

CHEN Lixue, et al. The mechanism of PTEN down-regulation on neuron intracellular free calcium and apoptosis. [J]. Journal of Chongqing Medical University (4):505-508(2006)
[1]陈力学,刘宝松,康格非,姜利人.PTEN表达下调对神经元胞内游离钙离子和神经元凋亡的研究[J].重庆医科大学学报,2006(04):505-508. DOI：

CHEN Lixue, et al. The mechanism of PTEN down-regulation on neuron intracellular free calcium and apoptosis. [J]. Journal of Chongqing Medical University (4):505-508(2006) DOI：

摘要

目的:探讨神经元缺血缺氧损伤再灌后

下调PTEN在阻断Ca2+内流及凋亡调控方面的保护机制。方法:建立神经元缺糖缺氧（oxygen-glucose deprivation

OGD）损伤模型

转染shRNAspten-GFP质粒

用Western blotting检测PTEN水平

Fura2-AM法测定胞内Ca2+浓度

流式细胞术和AO/EB检测神经元凋亡。结果:与正常组相比OGD后细胞凋亡率和胞内Ca2+浓度显著升高（P<0.05）;与对照组相比经shRNAspten干预后胞内Ca2+浓度和细胞凋亡率明显降低（P<0.05）。结论:shRNAspten-GFP能成功转染到神经元中

PTEN表达下调可阻断Ca2+内流并对缺糖缺氧诱导的细胞凋亡有拮抗作用

从而达到神经元保护的目的。

Abstract

Objective: To investigate the regulation of PTEN on apoptosis and the active roles in blocking Ca2+ influx and neuroprotection.Methods: In the OGD model with anaerobic gas mixture and deoxygenated

glucose-free extracellular solution

shRNAspten-GFP and unrelated control plasmid PshGFP transfection were done respectively.Intracellular free calcium concentration were measured with Fura-2 AM fluorescent indicator

the expression of PTEN were analyzed by immunoblotting(blot) and neuron apoptosis were detected by AO/EB and Flow Cytometry.Results: Apoptosis rate and calcium concentration after OGD was significantly higher than that of normal group(P<0.05).But intracellular free calcium concentration and apoptosis with shRNAspten-GFP treatment after OGD was significantly lower than that in OGD group and PshGFP group(P<0.05).Conclusion: shRNAspten-GFP plasmid can been expressed successfully in cultured neurons.PTEN down-regulation could effectively suppress apoptosis occurrence induced by OGD and the increase of intracellular free calcium concentration

and then protect neuron from anoxia injury.【

关键词

PTENshRNAspten-GFP质粒游离钙凋亡神经元培养

Keywords

PTENshRNAsCa2+apoptosisNeuron culture

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

TNF-α downregulates the expression of tight junction proteins ZO-1 and Claudin-5 in rat pulmonary microvascular endothelial cells

Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

XL413 inhibits colon cancer cell proliferation by inhibiting DNA replication and inducing cell apoptosis

3-methyladenine prevents sleep disturbance-induced liver injury by suppressing autophagy and endoplasmic reticulum stress

Related Author

Liu Jie

Xie Xingming

Niu Hongxia

Yang Xianzhi

Bai Jijia

Shi Guocui

Ma Shenmao

Ma Xigang

Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Department of Critical Care Medicine,General Hospital of Ningxia Medical University

Department of Respiratory Medicine,Cangzhou People's Hospital

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Gastroenterology, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

⁰