Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell

LI Xia; et al

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Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell

更新时间：2024-01-17

- Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell
- Journal of Chongqing Medical University Issue 5, Pages: 655-657(2006)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理生理教研室
  2. 重庆医科大学基础医学院病理生理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2006，
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[1]李霞,邓华瑜,陈黎.GA对乳腺癌MDA-MB-435s细胞端粒酶活性的影响[J].重庆医科大学学报,2006(05):655-657.

LI Xia, et al. Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell. [J]. Journal of Chongqing Medical University (5):655-657(2006)
[1]李霞,邓华瑜,陈黎.GA对乳腺癌MDA-MB-435s细胞端粒酶活性的影响[J].重庆医科大学学报,2006(05):655-657. DOI：

LI Xia, et al. Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell. [J]. Journal of Chongqing Medical University (5):655-657(2006) DOI：

摘要

目的:研究格尔德霉素（geldanamycin

GA）对乳腺癌细胞增殖和端粒酶活性的影响。方法:培养人乳腺癌细胞株MDA-MB-435s

应用MTT法检测GA对MDA-MB-435s细胞的生长抑制作用

流式细胞术分析细胞周期

用TRAP-ELISA法检测细胞端粒酶活性。结果:不同浓度GA对MDA-MB-435s细胞增殖有明显抑制作用

呈时效-量效依赖关系

GA作用后细胞呈现G2/M期阻滞

细胞端粒酶活性下降（P<0.05）。结论:GA对人乳腺癌MDA-MB-435s细胞增殖具有明显抑制作用

抑制端粒酶活性可能是其发挥抗癌作用的机制之一。

Abstract

Objective:To investigate the effect of GA on telomerase activity of breast cancer MDA-MB-435s cell.Methods:Human breast cancer cell line MDA-MB-435s was used in the study.Proliferation of MDA-MB-435s cells was measured with MTT assay.Cell cycle was analyzed by flow cytometry.The telomerase activity was measured by TRAP-ELISA assay.Results:GA inhibited the proliferation of MDA-MB-435s cells on a time-and dose-dependent manner.Aftertreatment of GA

MDA-MB-435s cells arrested at G2 /M phase.The telomerase activity of cells was decreasing compared with that of control cells(P<0.05).Conclusion:GA can prevent breast cancer MDA-MB-435s cells proliferation significantly

which may be related with decreasing the telomerase activity in cells.

关键词

乳腺癌GA增殖端粒酶

Keywords

breast cancerGAproliferationtelomerase

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Related Institution

Department of General Surgery,the Second Affiliated Hospital,Chongqing Medical University

the Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,Chongqing Medical University

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Department of Obstetrics and Gynecology,Yongchuan Hospital Affiliated to Chongqing Medical University

Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University

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