Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy

ZHENG Jianghua; et al

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Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy

更新时间：2024-01-17

- Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy
- Journal of Chongqing Medical University Issue 4, Pages: 482-485(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院血管外科
  2. 第三军医大学附属新桥医院普外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.35
- Published：2006，
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[1]郑江华,时德,赵渝,陈祖林.细胞外信号调节激酶通路激活抑制δ氨基酮戊酸-光动力疗法对SW480细胞的细胞毒作用[J].重庆医科大学学报,2006(04):482-485.

ZHENG Jianghua, et al. Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy. [J]. Journal of Chongqing Medical University (4):482-485(2006)
[1]郑江华,时德,赵渝,陈祖林.细胞外信号调节激酶通路激活抑制δ氨基酮戊酸-光动力疗法对SW480细胞的细胞毒作用[J].重庆医科大学学报,2006(04):482-485. DOI：

ZHENG Jianghua, et al. Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy. [J]. Journal of Chongqing Medical University (4):482-485(2006) DOI：

摘要

目的:探讨δ氨基酮戊酸-光动力疗法（ALA-PDT）诱导SW480细胞后的细胞外信号调节激酶（ERK）通路以及阻断此通路对细胞毒作用的影响。方法:将SW480细胞分为空白对照组、激光照射组、ALA组、ALA-PDT组（又分为30、60、90min组）及PD98059组

Western蛋白印迹检测各组细胞MEK和ERK1/2蛋白产物及磷酸化蛋白产物的表达;用MTT比色法分别检测各组细胞在不同时间段的光密度值

计算各组细胞存活率。结果:ALA-PDT后SW480细胞的ERK通路激活;阻断ERK通路后ALA-PDT对SW480细胞的细胞毒作用增强。结论:ERK通路的激活对ALA-PDT后SW480细胞起着保护作用;不同水平阻断ERK通路

可能成为增强ALA-PDT杀伤结肠癌细胞的新靶点。

Abstract

Objective: To investigate extracelluar signal-regulated kinase(ERK) pathway and cytotoxic effect after block of this pathway after 5-aminolevulinic acid(ALA)-based photodynamic therapy(PDT) on SW480 cell.Methods: SW480 was divided into control group

light group

ALA group

ALA-PDT group and PD98059 group.Western blot was used to detect the expression of protein products and phosphorylation protein products of MEK and ERK1/2.Optical density value and survival rate of each group were obtained at different time by MTT.Results: ERK pathway of SW480 cell was activated and block of this pathway increased cytotoxic effect of SW480 cell after ALA-PDT.Conclusions: Activation of ERK pathway protects SW480 cell from ALA-PDT.Block of ERK pathway at different levels may become a new target

which will enhance cytotoxic effect of ALA-PDT on colon carcinoma.

关键词

δ氨基酮戊酸光动力疗法:SW480细胞外信号调节激酶通路

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