Construction and identification of recombinant plasmid pQE30-hALRIP

DENG Jianchuan; et al

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Construction and identification of recombinant plasmid pQE30-hALRIP

更新时间：2024-01-17

- Construction and identification of recombinant plasmid pQE30-hALRIP
- Journal of Chongqing Medical University Issue 3, Pages: 229-231(2007)
- 作者机构：
  
  1. 重庆医科大学附属第二医院血液内科
  2. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2007，
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[1]邓建川,陈曜,孙航,刘杞.重组原核表达载体pQE30-hALRIP的构建及鉴定[J].重庆医科大学学报,2007(03):229-231+235.

DENG Jianchuan, et al. Construction and identification of recombinant plasmid pQE30-hALRIP. [J]. Journal of Chongqing Medical University (3):229-231(2007)
[1]邓建川,陈曜,孙航,刘杞.重组原核表达载体pQE30-hALRIP的构建及鉴定[J].重庆医科大学学报,2007(03):229-231+235. DOI：

DENG Jianchuan, et al. Construction and identification of recombinant plasmid pQE30-hALRIP. [J]. Journal of Chongqing Medical University (3):229-231(2007) DOI：

摘要

目的:构建人肝再生增强因子相互作用肽（hALR interacting protein

hALRIP）重组原核表达载体并进行鉴定。方法:用聚合酶链反应（PCR）扩增hALRIP编码区基因

将PCR扩增产物克隆至pMD18-T载体中并测序。利用pQE30质粒载体构建重组原核表达载体pQE30-hALRIP

并通过酶切电泳鉴定重组质粒。结果:构建了重组克隆载体pMD18-hALRIP和重组表达载体pQE30-hALRIP

测序及酶切鉴定与预计相同。结论:成功构建了重组原核表达载体pQE30-hALRIP

为进一步研究该多肽奠定基础。

Abstract

Objective:To construct a recombinant prokaryotic expression vector pQE30-hALRIP for further research.Method:PCR was used to amplify hALRIP codon region.The gene was inserted into pMD18-T vector and sequenced.Then the prokaryotic expression vector pQE30-hALRIP was constructed and identified. Results:Clonal recombinant vector pMD18- hALRIP and expression vector pQE30-hALRIP were constructed. DNA sequence analysis and restriction analysis showed both of the vectors were the same as the predicted results. Conclusion:Prokaryotic expression vector pQE30-hALRIP is successfully constructed.

关键词

人肝再生增强因子原核表达PQE30

Keywords

Human augmenter of liver regenerationprokaryotic expressionPQE30

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