Screening of proteins interacting with the Hepatitis B Virus PreS1 protein from T7-Phage display library

HUANG Ying; et al

您当前的位置：

首页 >

文章列表页 >

Screening of proteins interacting with the Hepatitis B Virus PreS1 protein from T7-Phage display library

更新时间：2024-01-17

- Screening of proteins interacting with the Hepatitis B Virus PreS1 protein from T7-Phage display library
- Journal of Chongqing Medical University Issue 4, Pages: 340-343(2007)
- 作者机构：
  
  1. 重庆医科大学附属第二医院感染科
  2. 重庆医科大学感染性疾病分子生物学教育部省部共建重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R450
- Published：2007，
扫描看全文
[1]黄英,张君,何茂锐,陈维贤,黄爱龙.用T7噬菌体展示技术筛选乙型肝炎病毒PreS1相互作用蛋白[J].重庆医科大学学报,2007(04):340-343.

HUANG Ying, et al. Screening of proteins interacting with the Hepatitis B Virus PreS1 protein from T7-Phage display library. [J]. Journal of Chongqing Medical University (4):340-343(2007)
[1]黄英,张君,何茂锐,陈维贤,黄爱龙.用T7噬菌体展示技术筛选乙型肝炎病毒PreS1相互作用蛋白[J].重庆医科大学学报,2007(04):340-343. DOI：

HUANG Ying, et al. Screening of proteins interacting with the Hepatitis B Virus PreS1 protein from T7-Phage display library. [J]. Journal of Chongqing Medical University (4):340-343(2007) DOI：

摘要

目的:用T7噬菌体筛选系统筛选乙型肝炎病毒PreS1的相互作用蛋白。方法:应用T7噬菌体展示技术

以通过原核表达的方式得到的乙型肝炎病毒PreS1作为靶分子

对噬菌体人肝细胞cDNA文库进行生物筛选

对筛选到的克隆进行DNA序列分析及同源性搜索。结果:经鉴定得到1个阳性克隆

确定了能与乙型肝炎病毒PreS1相互作用的蛋白可能为:细胞色素C氧化酶1（Cytochrome c Oxidase Subunit I

COX1）。结论:T7噬菌体展示筛选系统是筛选相互作用蛋白的一种简单、快速和有效的手段

筛选到的相互作用蛋白为进一步探讨PreS1的致病机制提供重要依据。

Abstract

Objective:To screen and identify proteins that interact with the hepatitis B virus PreS1 protein by means of T7-phage display system. Method:Hepatitis B virus PreS1 protein was expressed by prokaryotic expression and used as selected molecule to biopan the T7 select human liver cDNA library

the selected positive clones were identified by DNA sequence and analyzed with BLAST program in GenBank. Result:After BLAST in all positive clones

one protein:Cytochrome c Oxidase Subunit I(COX1)was found to interact with the hepatitis B virus PreS1 protein. Conclusion:T7-phage display system is a convenient

rapid and effective method for screening interacting proteins. This results will provide important evidences for studying the pathogenesis and mechanism of HBV.

关键词

T7噬菌体展示技术乙型肝炎病毒PreS1相互作用蛋白

Keywords

T7-phage displayHepatitis B virus PreS1 proteinInteracting proteins

references

Views

328

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰