Establishment and identification of hu-PBL-SCID mice model of human renal cell carcinoma

LIU Shihui; et al

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Establishment and identification of hu-PBL-SCID mice model of human renal cell carcinoma

更新时间：2024-01-17

- Establishment and identification of hu-PBL-SCID mice model of human renal cell carcinoma
- Journal of Chongqing Medical University Issue 11, Pages: 1143-1146(2007)
- 作者机构：
  
  1. 重庆医科大学附属第二医院泌尿外科
  2. 重庆医科大学附属第二医院泌尿外科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.11
- Published：2007，
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[1]刘世会,胡自力.人免疫重建荷人肾癌SCID小鼠模型的建立及其鉴定[J].重庆医科大学学报,2007(11):1143-1146.

LIU Shihui, et al. Establishment and identification of hu-PBL-SCID mice model of human renal cell carcinoma. [J]. Journal of Chongqing Medical University (11):1143-1146(2007)
[1]刘世会,胡自力.人免疫重建荷人肾癌SCID小鼠模型的建立及其鉴定[J].重庆医科大学学报,2007(11):1143-1146. DOI：

LIU Shihui, et al. Establishment and identification of hu-PBL-SCID mice model of human renal cell carcinoma. [J]. Journal of Chongqing Medical University (11):1143-1146(2007) DOI：

摘要

目的:建立具有人免疫学特性的肾癌SCID小鼠模型。方法:SCID小鼠腹腔注射人外周血淋巴细胞

皮下接种人肾癌细胞

观察小鼠的生物学及免疫学特性。结果:（1）免疫重建荷人肾癌组小鼠成瘤率为100%

较荷瘤组成瘤潜伏期显著延长、肿瘤体积明显缩小（P<0.01）;（2）第2、4、6周时小鼠外周血中人IgG分别为（394.86±16.70）μg/ml、（629.83±35.03）μg/ml、（994.96±70.11）μg/ml;（3）第6周小鼠外周血中人CD3+T淋巴细胞为（12.31±0.86）%;（4）免疫组化检测小鼠肿瘤和脾脏中存在人CD3+T淋巴细胞。结论:成功建立免疫重建荷人肾癌SCID小鼠模型

为肾癌治疗的研究提供了理想动物模型。

Abstract

Objective:To investigate the feasibility of establishing a human renal cell carcinoma model in human peripheral blood lymphocyte-engrafted to severe combined immunodeficient(hu-PBL-SCID)mice.Methods:The biological and immunological features of mice were evaluated after intra-peritoneal injection of human peripheral blood lymphocytes(PBL)and subcutaneous implantation of human renal cell carcinoma cells(RCCCs).Results:(1)Subcutaneous tumors developed in all the mice given human PBL and RCCCs.The latency period was significantly prolonged

and the tumor size was markedly depressed

as compared with the mice given RCCCs(P<0.01).(2)In the 2nd

4th and 6th week

human IgG in the murine peripheral blood of the hu-RCCC-PBL-SCID model were(394.86±16.70)μg/ml、(629.83±35.03)μg/ml and(994.96±70.11)μg/ml

respectively.(3)In the 6th week the human CD3+T lymphocytes was(12.31±0.86)%.(4)Immunohistochemical staining revealed presence of remarkable human CD3+T lymphocytes in murine tumor and spleen.Conclusion:A human renal cell carcinoma model has been established in hu-PBL-SCID mice which is an ideal animal model for preclinical research and treatment for renal cell carcinoma.

关键词

肾癌SCID小鼠免疫重建动物模型

Keywords

renal cell carcinomaSCID miceImmune reconstructonAnimal model.

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