The responses of microvascular endothelial cells from human ovary carcinoma to PDGF-BB

YANG Ying; et al

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The responses of microvascular endothelial cells from human ovary carcinoma to PDGF-BB

更新时间：2024-01-17

- The responses of microvascular endothelial cells from human ovary carcinoma to PDGF-BB
- Journal of Chongqing Medical University Issue 8, Pages: 808-811(2007)
- 作者机构：
  
  1. 第三军医大学新桥医院妇产科
  2. 第三军医大学新桥医院妇产科,重庆,400037
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.31
- Published：2007，
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[1]杨鹰,阎萍,韩静,连艳.人卵巢癌微血管内皮细胞对PDGF-BB刺激的反应[J].重庆医科大学学报,2007(08):808-811.

YANG Ying, et al. The responses of microvascular endothelial cells from human ovary carcinoma to PDGF-BB. [J]. Journal of Chongqing Medical University (8):808-811(2007)
[1]杨鹰,阎萍,韩静,连艳.人卵巢癌微血管内皮细胞对PDGF-BB刺激的反应[J].重庆医科大学学报,2007(08):808-811. DOI：

YANG Ying, et al. The responses of microvascular endothelial cells from human ovary carcinoma to PDGF-BB. [J]. Journal of Chongqing Medical University (8):808-811(2007) DOI：

摘要

目的:探讨人卵巢癌微血管内皮细胞与HUVEC在生物学特性及对血管生成因子刺激反应的差异。方法:分离纯化人卵巢癌微血管内皮细胞

以HUVEC为对照

用PDGF-BB刺激两种内皮细胞

利用免疫细胞化学和RT-PCR技术观察刺激后两种内皮细胞增殖、PCNA及VEGF表达的变化。结果:PDGF-BB（20ng/ml）能促进两种内皮细胞的增殖、PCNA和VEGF的表达

人卵巢癌微血管内皮细胞增殖和PCNA表达增强的幅度弱于HUVEC;而VEGF蛋白及mRNA表达增强的幅度高于HUVEC（P<0.05）。结论:人卵巢癌微血管内皮细胞与HUVEC对PDGF-BB刺激的反应有显著差异

为深入研究卵巢癌血管生成机制和抗血管生成治疗提供了实验模型。

Abstract

Objective:To investigate the difference of biological characteristics and responses to PDGF-BB between human ovarian carcinoma-derived microvascular endothelial cells and human umbilical vein endothelial cells.Methods:The ovarian carcinoma-derived microvascular endothelial cells were isolated and purifed from ovarian carcinoma tissues.Compared to the human umbilical vein endothelial cells

the biological characteristics and responses to PDGF-BB were observed in OdMEC for the changes of growth

PCNA and VEGF expression.Results:20ng/ml PDGF-BB could enhance both kinds of endothelial cells to proliferate

express PCNA and VEGF.The proliferation and PCNA protein expression in HUVEC were augmented more significantly than those in OdMEC

the protein and mRNA of VEGF expression were augmented more significantly than those in HUVEC.Conclusion:The responses of OdMEC to PDGF-BB were significantly different from those of HUVEC

which provides experiment basis for research on ovarian carcinoma vascularization and antiangiogenesis therapy.

关键词

卵巢癌血管内皮细胞细胞培养血小板源性生长因子

Keywords

ovarian carcinomavascular endothelial cellcell culturePlatelet-derived growth factor

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