Effects of high glucose concentration on MMP-2 expression and cell cycle in cultured human umbilical vein endothelial cells

PENG Hui; et al

您当前的位置：

首页 >

文章列表页 >

Effects of high glucose concentration on MMP-2 expression and cell cycle in cultured human umbilical vein endothelial cells

更新时间：2024-01-17

- Effects of high glucose concentration on MMP-2 expression and cell cycle in cultured human umbilical vein endothelial cells
- Journal of Chongqing Medical University Issue 8, Pages: 830-834(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院眼科
  2. 重庆医科大学附属第一医院耳鼻喉科
  3. 重庆医科大学组织胚胎学教研室
  4. 重庆医科大学附属第一医院眼科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R774.1
- Published：2007，
扫描看全文
[1]彭惠,洪苏玲,李平华,李静,周希,张黎.高糖对人脐静脉内皮细胞基质金属蛋白酶及细胞周期的影响[J].重庆医科大学学报,2007(08):830-834.

PENG Hui, et al. Effects of high glucose concentration on MMP-2 expression and cell cycle in cultured human umbilical vein endothelial cells. [J]. Journal of Chongqing Medical University (8):830-834(2007)
[1]彭惠,洪苏玲,李平华,李静,周希,张黎.高糖对人脐静脉内皮细胞基质金属蛋白酶及细胞周期的影响[J].重庆医科大学学报,2007(08):830-834. DOI：

PENG Hui, et al. Effects of high glucose concentration on MMP-2 expression and cell cycle in cultured human umbilical vein endothelial cells. [J]. Journal of Chongqing Medical University (8):830-834(2007) DOI：

摘要

目的:探讨高浓度葡萄糖作用于脐静脉内皮细胞后

对脐静脉血管内皮细胞系的基质金属蛋白酶（MMP-2）表达、超微结构及细胞周期的影响

为阐明高血糖在糖尿病血管病变、糖尿病视网膜病变的早期发病中作用与机制提供依据。方法:在高浓度葡萄糖条件下培养人脐静脉内皮细胞

采用RT-PCR、Western-blot法检测细胞基质金属蛋白酶（MMP-2）mRNA和蛋白表达情况、电镜观察超微结构及流式细胞仪检测细胞周期的变化。结果:高浓度葡萄糖可使内皮细胞MMP-2表达增加、细胞增生活跃

细胞出现肿胀

体积增大

细胞质溶解、核溶解

异染色质边集

内质网扩张

出现固缩线粒体、环形线粒体

髓样结构等改变。结论:高糖诱导的MMP-2mRNA和蛋白表达增加

可能在糖尿病视网膜病变的早期发病中起重要作用。

Abstract

Objective:To examine the effects of glucose on the expression of matrix metalloproteinases-2(MMP-2)and the changes of ultramicrostructure

cell cycle in cultured human umbilical vein endothelial cells.Methods:Human umbilical vein endothelial cells(hUVEC)were incubated in a culture medium with 11.2mmol/L

16.8mmol/L and 33.6mmol/L glucose concentrations for 24h

48h

72h respectively.The expression of MMP-2 was measured by RT-PCR and Western-blot.Results:When D-glucose concentrations rose

hUVEC exposed to high glucose concentration(11.2mmol/L

16.8mmol/L and 33.6mmol/L)increased in cell expression of MMP-2 after 48 h and 72 h of exposure

as compared with those cultured in medium with a low glucose concentration(5.6mmol/L).And cell cycle changed too.Conclusion:High concentration of glucose can arrest the proliferative response and show that even a short-term exposure of endothelial cells(ECs)to high glucose concentration leads to their activation associated with increased expression of MMP-2 and that this effect may appear more significantly along with time of exposure to high concentration of glucose.

关键词

血管内皮细胞葡萄糖浓度MMP-2糖尿病视网膜病变

Keywords

vascular endothelial cellGlucose concentrationsMMP-2diabetic retinopathy

references

Views

174

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Primary study on the mechanism of intralesional therapy for hemangioma with glucocorticoid

Significance of TGF-β₂ expression in the retina of diabetic hamster

Expressions and their Relationship of VEGF and MM P - 2 in Pituitary Adenomas

Construction and expression of enhanced green fluorescent protein and HGF co-expression vector

The responses of microvascular endothelial cells from human ovary carcinoma to PDGF-BB

Related Author

唐开勇

李静

李晓庆

王珊

周德凯

et al

LIU Qian

姜蓉

Related Institution

重庆医科大学儿童医院病理科

重庆医科大学基础医学院组胚教研室

Department of General Surgery,Children’s Hospital,Chongqing Medical University

重庆医科大学附属第一医院神经外科

⁰