In vitro study on the effect of bone marrow mesenchymal stem cells on the fatty acid metabolism of hypertrophic cardiomyocytes

ZHUO Wen-yu; et al

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In vitro study on the effect of bone marrow mesenchymal stem cells on the fatty acid metabolism of hypertrophic cardiomyocytes

更新时间：2024-01-17

- In vitro study on the effect of bone marrow mesenchymal stem cells on the fatty acid metabolism of hypertrophic cardiomyocytes
- Journal of Chongqing Medical University Issue 2, Pages: 129-132(2008)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院心血管内科
  2. 重庆医科大学附属儿童医院儿科医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R541
- Published：2008，
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[1]卓文玉,陈沅,田杰,朱静,向平.骨髓间充质干细胞对肥大心肌细胞脂肪酸代谢影响的体外研究[J].重庆医科大学学报,2008(02):129-132+165.

ZHUO Wen-yu, et al. In vitro study on the effect of bone marrow mesenchymal stem cells on the fatty acid metabolism of hypertrophic cardiomyocytes. [J]. Journal of Chongqing Medical University (2):129-132(2008)
[1]卓文玉,陈沅,田杰,朱静,向平.骨髓间充质干细胞对肥大心肌细胞脂肪酸代谢影响的体外研究[J].重庆医科大学学报,2008(02):129-132+165. DOI：

ZHUO Wen-yu, et al. In vitro study on the effect of bone marrow mesenchymal stem cells on the fatty acid metabolism of hypertrophic cardiomyocytes. [J]. Journal of Chongqing Medical University (2):129-132(2008) DOI：

摘要

目的:探讨骨髓间充质干细胞对肥大心肌细胞脂肪酸代谢的影响及其机制。方法:体外培养的Wistar新生大鼠心肌细胞随机分为3组:正常对照组、血管紧张素Ⅱ（AngⅡ）作用组、骨髓间充质干细胞干预组

采用免疫荧光显微镜测定心肌细胞核因子NF-κB和过氧化物酶体增殖物激活受体（PPARs）PPARγ、PPARα的活性

紫外分光光度法检测心肌细胞内游离脂肪酸（FFA）含量。结果:AngⅡ刺激乳鼠心肌细胞NF-κB活性增强

抑制PPARα和PPARγ活性减弱

增加心肌细胞内FFA蓄积（P<0.01）;MSCs干预组心肌细胞NF-κB活性及心肌细胞内FFA含量较AngⅡ作用组显著降低（P<0.01和P<0.01）而PPARα和PPARγ活性增强（P<0.05和P<0.01）。结论:骨髓间充质干细胞可改善肥大心肌细胞的脂肪酸代谢

这可能是骨髓间充质干细胞移植改善心功能作用机制之一

NF-κB及PPARs可能是其作用的信号通路之一。

Abstract

Objectives:To inverstigate the effect of bone marrow mesenchymal stem cells(MSCs) on the fatty acid metabolism of hypertrophic cardiomyocytes and disclosure its mechanisms.Methods:Wistar neonate rat cardiomyocytes cultured in vitro were randomized into an angiotensin(Ang)-Ⅱstimulating group(n=5)

a MSCs treated Ang-Ⅱ stimulating group(Intervention group

n=5) and a normal control group(n=5).The activity of nuclear factor(NF)-κB and peroxisome proliferators-activated receptor(PPAR) α and γ were detected by immunofluorescence microscope

and intracellular free fatty acids(FFA) content by ultraviolet spectrophotometry.Results:Ang-Ⅱstimulated the NF-κB activation of neonate rat cardiomyocytes to strengthen

inhibited the activation of PPARα and PPARγ to weaken and increased the accumulation of FFA(P<0.01).For MSCs intervention group

the activation of NF-κB and the accumulation of FFA decreased significantly(P<0.01 and P<0.01) while the activation of PPARα and PPARγ were up-regulated compared with the Ang-Ⅱstimulating group(P<0.05 and P<0.01).Conclusion:MSCs could improve the fatty acid metabolism of hypertrophic cardiomyocytes

which is probably one of the mechanisms of the marrow mesenchymal stem cell transplantation improving the heart functions

and NF-κB and PPARs may be the signal passage of function.

关键词

骨髓间充质干细胞心肌细胞NF-κB游离脂肪酸

Keywords

bone marrow mesenchymal stem cellcardiomyocyteNuclear factor κBFree fatty acids

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