Identification and cultivation of neural stem cells in neonatal rats in vitro

YANG Qin; et al

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Identification and cultivation of neural stem cells in neonatal rats in vitro

更新时间：2024-01-17

- Identification and cultivation of neural stem cells in neonatal rats in vitro
- Journal of Chongqing Medical University Issue 11, Pages: 1134-1137(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室重庆医科大学基础研究所
  2. 重庆医科大学附属第一医院影像科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2007，
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[1]杨琴,曾志磊,谢鹏,杨军,吕发金.新生大鼠神经干细胞的体外培养和鉴定[J].重庆医科大学学报,2007(11):1134-1137.

YANG Qin, et al. Identification and cultivation of neural stem cells in neonatal rats in vitro. [J]. Journal of Chongqing Medical University (11):1134-1137(2007)
[1]杨琴,曾志磊,谢鹏,杨军,吕发金.新生大鼠神经干细胞的体外培养和鉴定[J].重庆医科大学学报,2007(11):1134-1137. DOI：

YANG Qin, et al. Identification and cultivation of neural stem cells in neonatal rats in vitro. [J]. Journal of Chongqing Medical University (11):1134-1137(2007) DOI：

摘要

目的:探讨新生大鼠神经干细胞的分离培养和鉴定。方法:采用无血清细胞培养技术

间接免疫细胞化学染色法。结果:新生大鼠脑组织分离的细胞具有连续传代和增殖能力

表达神经上皮干细胞蛋白

诱导分化后的细胞表达神经元细胞、星形胶质细胞、少突胶质细胞特异性蛋白。结论:该方法分离培养的神经干细胞保持了干细胞的未分化属性

具有自我更新和多项分化潜能。

Abstract

Objective:To study the identification and cultivation of neural stem cells in neonatal rats in vitro.Methods:Cell culture in serum-free medium

indirect immunofluorescence cytochemistry were used.Results:The neural stem cells isolated in newborn rats had the potential of forming clones and serial passage

expressing neuroepithelial stem cell protein(nestin)and differentiating into neurons and astrocytes and oligodendrocytes.Conclusion:Neural stem cells maintain their undifferentiated features and have the capacity of self-renewal

proliferation and pluripotentiality.

关键词

神经干细胞细胞培养新生大鼠

Keywords

neural stem cellscell cultureneonatal rat

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