Preparation of IL-2 conjugated immunoliposomes and study on its stability

ZHU Li; et al

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Preparation of IL-2 conjugated immunoliposomes and study on its stability

更新时间：2024-01-17

- Preparation of IL-2 conjugated immunoliposomes and study on its stability
- Journal of Chongqing Medical University Issue 8, Pages: 1000-1002(2008)
- 作者机构：
  
  重庆医科大学药学院药剂教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R94
- Published：2008，
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[1]朱莉,胡春梅,赵俊义,潘黎军,王驰.IL-2免疫靶向脂质体的制备及稳定性研究[J].重庆医科大学学报,2008(08):1000-1002.

ZHU Li, et al. Preparation of IL-2 conjugated immunoliposomes and study on its stability. [J]. Journal of Chongqing Medical University (8):1000-1002(2008)
[1]朱莉,胡春梅,赵俊义,潘黎军,王驰.IL-2免疫靶向脂质体的制备及稳定性研究[J].重庆医科大学学报,2008(08):1000-1002. DOI：

ZHU Li, et al. Preparation of IL-2 conjugated immunoliposomes and study on its stability. [J]. Journal of Chongqing Medical University (8):1000-1002(2008) DOI：

摘要

目的:寻求一种简便、快速、结合率高的制备白介素-2（Interleukin-2

IL-2）免疫脂质体的方法。方法:分别用戊二醛法、十一醛法、衍生化法制备IL-2免疫脂质体。对上述几种制备方法进行分析比较

Bradford比色法测定IL-2的结合效率;离心加速实验考察脂质体稳定性。结果:十一醛法通过席夫碱一对一的结合IL-2和脂质体

不会引起IL-2、脂质体自身相互交联

结合率高

稳定性好。结论:拟采用十一醛法作为后期细胞实验中IL-2免疫脂质体的制备方法。

Abstract

Objective:To search for a simple and rapid method of preparating the IL-2 conjugated immunoliposomes with high conjugate rate.Methods:The IL-2 conjugated immunoliposomes were prepared by Glutaraldhyde

Undecanal or Derivatization.All methods were analyzed and compared.The conjugate rate of IL-2 was determined by Bradford Assay.Centrifugal acceleration experiment served to prove the liposome stability.Results:IL-2 conjugated liposome by shiff’s base through Undecanal didn’t cause IL-2-self or liposme-self inter-cross link

and conjugate rate was high and stability was good.Conclution:Undecanal is suggested to prepare the IL-2 conjugated immunoliposomes for the anaphase cell experiment.

关键词

IL-2免疫脂质体制备结合率稳定性

Keywords

IL-2ImmunoliposomePreparationConjugate ratestability

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