An immunohistochemical study on the time-dependent expression of MIP-1αduring healing of skin incised wound in mice

LIAO Ying; et al

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An immunohistochemical study on the time-dependent expression of MIP-1αduring healing of skin incised wound in mice

更新时间：2024-01-17

- An immunohistochemical study on the time-dependent expression of MIP-1αduring healing of skin incised wound in mice
- Journal of Chongqing Medical University Issue 9, Pages: 1067-1070(2008)
- 作者机构：
  
  重庆医科大学基础医学院法医教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： D919
- Published：2008，
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[1]廖鹰,邓世雄.小鼠皮肤切创愈合过程中巨噬细胞炎性蛋白-1α的表达及其与损伤时间的关系[J].重庆医科大学学报,2008(09):1067-1070.

LIAO Ying, et al. An immunohistochemical study on the time-dependent expression of MIP-1αduring healing of skin incised wound in mice. [J]. Journal of Chongqing Medical University (9):1067-1070(2008)
[1]廖鹰,邓世雄.小鼠皮肤切创愈合过程中巨噬细胞炎性蛋白-1α的表达及其与损伤时间的关系[J].重庆医科大学学报,2008(09):1067-1070. DOI：

LIAO Ying, et al. An immunohistochemical study on the time-dependent expression of MIP-1αduring healing of skin incised wound in mice. [J]. Journal of Chongqing Medical University (9):1067-1070(2008) DOI：

摘要

目的:探讨MIP-1α在小鼠皮肤切创损伤愈合过程中的表达分布变化规律及其与损伤时间的关系。方法:小鼠背部制做全层切创

应用免疫组织化学技术及阳性细胞计数法观察伤后小鼠切创组织中不同时程的生前伤（1h～14d）和死后伤（0.5～6h）皮肤创伤局部MIP-1α的表达

并和无切创的小鼠作为对照。结果:在生前损伤组中

MIP-1α最早在伤后6h出现阳性表达

随后表达逐渐增多

在3d达到高峰

其后呈下降趋势。而MIP-1α在早期死后伤组及对照组仅在表皮

皮脂腺

汗腺上皮细胞呈弱阳性表达。结论:MIP-1α在小鼠皮肤切创愈合过程中呈现一定时序性变化

有望作为法医损伤时间推断的一个有用的参考指标。

Abstract

Objective:To explore the expression of MIP-1α during healing of skin incised wound and the applicability of time-dependent expressions of MIP-1α to determination of wound age. Methods:An incised wound was made in the dorsal skin of mouse. Immunohistochemical and image-analysis techniques were employed in vital skin wounds 1h~14d after incision and postmortem incision 0.5~6h after death

and the non-incised mouse skin was used as contro1.Results:In the vital skin incisions

expression of MIP-1α began at 6h after incision

which increased subsequently

and peaked at 3d.The quantity of MIP-1αexpression decreased and minimized in the specimens aged 14 days. In the non-wounded groups and postmortem incision groups MIP-1α was only detected as weak expression in the epidermic cells

sweat gland cells and endothelial cells. Conclusion:The time-dependent expression of MIP-1αduring healing of skin incised wound may be used as a useful reference marker.

关键词

MIP-1α趋化因子损伤时间切创免疫组化

Keywords

MIP-1αChemokinesWounding interval estimationIncised woundimmunohistochemistry

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Related Institution

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