Inhibition of survivin expression by RNA interference in renal cancer cell line 786-O

XU Bin; et al

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Inhibition of survivin expression by RNA interference in renal cancer cell line 786-O

更新时间：2024-01-17

- Inhibition of survivin expression by RNA interference in renal cancer cell line 786-O
- Journal of Chongqing Medical University Issue 9, Pages: 1080-1083(2008)
- 作者机构：
  
  重庆医科大学附属第二医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.11
- Published：2008，
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[1]徐斌,胡自力.RNA干扰技术特异性抑制肾癌survivin表达的实验研究[J].重庆医科大学学报,2008(09):1080-1083.

XU Bin, et al. Inhibition of survivin expression by RNA interference in renal cancer cell line 786-O. [J]. Journal of Chongqing Medical University (9):1080-1083(2008)
[1]徐斌,胡自力.RNA干扰技术特异性抑制肾癌survivin表达的实验研究[J].重庆医科大学学报,2008(09):1080-1083. DOI：

XU Bin, et al. Inhibition of survivin expression by RNA interference in renal cancer cell line 786-O. [J]. Journal of Chongqing Medical University (9):1080-1083(2008) DOI：

摘要

目的:体外转录合成survivin siRNA（Small interference RNA

siRNA）

观察其在肾癌786-O细胞株中抑制survivin的表达情况。方法:体外转录合成3组survivin序列特异性双链RNA（Double-stranded RNA

dsRNA）

转染786-O细胞株中

用RT-PCR和Western blot检测转染后survivin基因的mRNA和蛋白的表达

MTT法检测细胞活性。结果:有2组转染特异性siRNA的786-O细胞表达survivin mRNA及蛋白均下调

活性受到抑制

另一组效果不明显。结论:体外转录合成特异性survivin siRNA能有效抑制肾癌786-O细胞中survivin的表达

抑制细胞的活性

RNA干扰（RNA interfering

RNAi）技术可能为肾癌的基因治疗提供一种新策略。

Abstract

Objective:To study the inhibition of survivin expression by small interference RNA(siRNA) synthesized in vitro in renal cancer cell line 786-O. Methods:Three groups of survivin siRNAs were chemically synthesized in vitro and then transfected into cell line 786-O. The expression of survivin mRNA and protein after transfection were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western Blotting. The proliferation of cells was detected by MTT. Results:Two sequence specific siRNAs targeting survivin down-regulated the expression of survivn mRNA and protein. The proliferation of cells was inhibited after transfection. The third one was ineffective. Conclusion:Sequence specific siRNA targeting survivin can efficiently inhibit the survivin expression and cell proliferation in renal cancer cell line 786-O. The successful application of RNAi targeting survivin might extend the list of available gene therapeutic modalities in renal cancer.

关键词

肾癌基因治疗RNA干扰生存素

Keywords

renal cancergene therapyRNA interferencesurvivin

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Related Institution

Department of Urinary Surgery，The First Affiliated Hospital of Chongqing Medical University

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Teaching and Research Section of Pathology,College of Basic Medicine,Molecular Medicine and Cancer Research Center,Chongqing Medical University

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