Determination of cisatracurium in the plasma of rabbits by HPLC coupled with fluorescence detection

ZHOU Ding-li; et al

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Determination of cisatracurium in the plasma of rabbits by HPLC coupled with fluorescence detection

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- Determination of cisatracurium in the plasma of rabbits by HPLC coupled with fluorescence detection
- Journal of Chongqing Medical University Issue 6, Pages: 724-725(2008)
- 作者机构：
  
  1. 重庆医科大学药学院药物分析教研室
  2. 重庆青阳药业有限公司
  3. 重庆医科大学药学院药物分析教研室,重庆,400016
  4. ,重庆,400025
  5. 重庆医科大学医药研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R96
- Published：2008，
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[1]周定利,钟玲,邱妍川,雷光洪,尚京川.兔血浆中顺式阿曲库铵的HPLC-荧光法测定[J].重庆医科大学学报,2008(06):724-725+757.

ZHOU Ding-li, et al. Determination of cisatracurium in the plasma of rabbits by HPLC coupled with fluorescence detection. [J]. Journal of Chongqing Medical University (6):724-725(2008)
[1]周定利,钟玲,邱妍川,雷光洪,尚京川.兔血浆中顺式阿曲库铵的HPLC-荧光法测定[J].重庆医科大学学报,2008(06):724-725+757. DOI：

ZHOU Ding-li, et al. Determination of cisatracurium in the plasma of rabbits by HPLC coupled with fluorescence detection. [J]. Journal of Chongqing Medical University (6):724-725(2008) DOI：

摘要

目的:建立以反相高效液相色谱-荧光法测定兔血浆中顺式阿曲库铵（Cisatracurium）浓度的方法。方法:色谱柱为Agilent Hypersil ODS-C18柱（5μm

4.0mm×125mm）

流动相为50mmol/L磷酸二氢钾缓冲液（pH3.0）-乙腈（50∶50）

荧光检测的激发波长200nm、发射波长320nm。结果:顺式阿曲库铵检测浓度在25～2000ng/ml线性关系良好

r=0.9992。最低检测限为25ng/ml

日内精密度RSD≤2.56%

日间精密度RSD≤3.68%;提取回收率为85.2%～86.1%（n=5）

加样回收率为93.4%～98.8%。结论:本方法简便

灵敏度高

重现性好

可满足药代动力学研究的需要。

Abstract

Objective:To determinate the concentration of Cisatracurium in the plasma of an rabbit via a proposed HPLC method. Methods:A C18 column was used

the mobile phase was made up of 50mmol/L dipotassium hydrogen phosphate(pH=3.0) and acetonitrile(50∶50)

and examination and emission wavelengths are 200nm and 320nm

respectively. Results:the linear range was 25~2 000ng/ml

the limit of detection(LOD) is 25ng/ml

the intra- and inter-precisions(RSD) were lower than2.56% and 3.68%

respectively. Besides

the recovery of extraction was 85.2%~86.1%(n=5) and the recovery of addition was 93.4%~98.8%. Conclusion:The proposed method proved to be accurate and repetitive.

关键词

顺式阿曲库铵血药浓度药代动力学高效液相色谱

Keywords

CisatracuriumPlasma concentrationpharmacokineticsHPLC

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