Expression of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock

WU Bin; et al

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Expression of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock

更新时间：2024-01-17

- Expression of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock
- Journal of Chongqing Medical University Issue 2, Pages: 173-176(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院麻醉科
  2. 重庆医科大学附属第二医院麻醉科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R459.7
- Published：2008，
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[1]吴彬,但伶.CD40/CD40L在兔失血性休克肺组织中的表达[J].重庆医科大学学报,2008(02):173-176.

WU Bin, et al. Expression of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock. [J]. Journal of Chongqing Medical University (2):173-176(2008)
[1]吴彬,但伶.CD40/CD40L在兔失血性休克肺组织中的表达[J].重庆医科大学学报,2008(02):173-176. DOI：

WU Bin, et al. Expression of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock. [J]. Journal of Chongqing Medical University (2):173-176(2008) DOI：

摘要

目的:探讨CD40/CD40L在兔失血性休克肺组织中的表达及作用。方法:选取健康家兔18只

随机分为正常对照组（A组）

失血性休克1h组（B组）和失血性休克2h组（C组）

每组6只。除对照组外

其余动物通过Wiggers改良法制作失血性休克肺损伤模型。采用酶联免疫吸附试验（ELISA法）测定血浆中TNF-α、IL-10浓度

免疫组织化学法检测CD40/CD40L在肺组织中的表达

观察肺组织含水量及肺组织病理学变化。结果:与对照组相比

B、C组肺组织可见明显肺损伤病理改变

肺含水量增加（P<0.05）

且血浆TNF-α、IL-10含量较A组明显增高（P<0.01）

休克2h后血浆中TNF-α含量较休克1h血浆中含量明显增高（P<0.01）;免疫组化结果显示失血性休克后肺组织中可见CD40、CD40L在肺泡上皮细胞及支气管粘膜上皮细胞表达

而正常肺组织内少见或未见表达。结论:失血性休克后肺损伤的发生可能与CD40/CD40L的异常表达有关。

Abstract

Objective:To investigate the expression and the effects of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock.Methods:18 healthy rabbits were randomly divided into control group(group A)

hemorrhagic shock 1 hour group(group B) and hemorrhagic shock 2 hours group(group C)

with 6 rabbits in each group.Except the rabbits in the control group

rest rabbits were made into hemorrhagic shock models with lung injury according to Wiggers modified method.TNF-α、IL-10 in plasma were measured by ELISA.Expressions of CD40 and CD40L in lung tissue were measured by immunohistohemistry.Water quantity and pathological change in the lung were observed.Results:The lung of group B and C displayed obvious injury

water quantity increased(P<0.05).Compared with group A

plasma concentration of TNF-α and IL-10 in group B and C increased obviously(P<0.01)

and the concentration of TNF-α at the 2nd hour of hemorrhagic shock increased obviously than at the 1st hour(P<0.01).The immunohistohemistry results revealed that CD40 and CD40L expressed in the lung tissue after hemorrhagic shock but not in the normal.Conclusion:The alteration of CD40 and CD40L expression may participate in the generation and development of lung injury during hemorrhagic shock.

关键词

失血性休克CD40CD40L肺

Keywords

Hemorrhagic shockCD40CD40Llung

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