Study on the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine against echinococcus multilocularis

LI Wen-gui; et al

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Study on the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine against echinococcus multilocularis

更新时间：2024-01-17

- Study on the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine against echinococcus multilocularis
- Journal of Chongqing Medical University Issue 1, Pages: 9-12(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院传染病寄生虫病研究所
  2. 重庆医科大学附属第一医院传染病寄生虫病研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2008，
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[1]李文桂,王鸿,朱佑明.多房棘球绦虫重组BCG-Em14-3-3疫苗诱导小鼠脾细胞因子变化的研究[J].重庆医科大学学报,2008(01):9-12.

LI Wen-gui, et al. Study on the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine against echinococcus multilocularis. [J]. Journal of Chongqing Medical University (1):9-12(2008)
[1]李文桂,王鸿,朱佑明.多房棘球绦虫重组BCG-Em14-3-3疫苗诱导小鼠脾细胞因子变化的研究[J].重庆医科大学学报,2008(01):9-12. DOI：

LI Wen-gui, et al. Study on the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine against echinococcus multilocularis. [J]. Journal of Chongqing Medical University (1):9-12(2008) DOI：

摘要

目的:探讨多房棘球绦虫重组BCG-Em14-3-3疫苗免疫和Em原头节攻击后小鼠脾细胞因子的变化。方法:将疫苗采用皮下注射和鼻腔内接种分别免疫BALB/c鼠

免疫后8周用多房棘球绦虫原头节进行攻击感染

感染后18周杀鼠取脾

分离脾细胞

用EmAg或ConA刺激培养

收集脾细胞培养上清液

用试剂盒检测脾细胞培养上清液的IL-2I、FN-γ、TNF-α和IL-4水平

同时设有空载体、BCG和PBS对照。结果:疫苗接种组的IFN-γ和TNF-α水平升高

IL-4水平降低;鼻腔内接种组的TNF-α水平高于皮下注射组。结论:多房棘球绦虫重组BCG-Em14-3-3疫苗诱导小鼠产生一个TH1型反应

从而对抗Em原头节攻击感染。

Abstract

Objective:To investigate the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em) and challenge with Em protoscoleces.Methods:BALB/c mice were subcutaneously and intranasally vaccinated respectively

then challenged with Em protoscoleces at 8th week of vaccination and killed at 18th week of infection to get spleen.Splenocytes were separated to culture by stimulation with EmAg or ConA.The supernatants were collected to measure IL-2、IFN-γ、TNF-αand IL-4 by ELISA Kits

and blank vector

BCG and PBS served as control.Results:In the groups of immunization

levels of IFN-γand TNF-α increased

but that of IL-4 decreased.The level of TNF-α in the intranasal group was higher than that in the subcutaneous group.Conclusion:TH1 response is induced in mice by rBCG-Em14-3-3 vaccine which is against the challenge of Em protoscoleces.

关键词

多房棘球绦虫重组BCG-Em14-3-3疫苗细胞因子

Keywords

Echinococcus multilocularisRecombinant BCG-Em14-3-3 vaccinecytokines

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